Recombinant antibody HCV 1b NS5B (clone R9A2)

Catalogue #

C2-424-100

Name

Recombinant antibody HCV 1b NS5B (clone R9A2)

Target

HCV 1b NS5B

Target Description

Recombinant Hepatitis C Virus subtype 1b non-structural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)

Uniprot ID

Q9WMX2

Clonality

Human monoclonal

Clone

R9A2

Class

hIgG1

Reactivity

Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT)

Application

ELISA, WB, IF

Protocol

ELISA:

5 - 10 µg/ml

WB:

1 µg/ml under reducing conditions.

IF:

dilution 1:1000

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Related Products

This antibody is derived from A2-424-100 mouse monoclonal antibody to HCV 1b NS5B, clone 9A2.

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. Antibody is derived from mouse anti-HCV NS5B monoclonal antibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence.