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Antibody development and engineering

From antibody humanization and reformatting to afucosylated bispecific antibodies – we have developed and produced them all. Our technology and experience gives us an ability to discover antibodies against difficult multi-pass transmembrane proteins. 

Icosagen provides antibody discovery and development services, using proprietary technologies. 

Bispecific antibody development

Our services cover the entire spectrum of bispecific and multispecific antibodies or similar antibody-like molecules from discovery to production and performance or structural engineering. Throughout this process, we employ in vivo and in vitro antibody discovery platforms, custom analytics and individually crafted purification methods for each molecule, ensuring the final product meets set requirements.  

To optimize therapeutic potential of a selected lead candidate, we can enhance or decrease its affinity, improve binding specificity to prevent off-target effects, mitigate aggregation and reduce immunogenicity through humanization. Further, we can modulate its half-life, immune cell binding and activation, and meticulously examine each molecule using tailored in vitro and in vivo assays. 

As a more straightforward approach, we can produce and purify antibodies based on electronic sequences provided by our clients, without any further optimizations. 

Why choose Icosagen for bispecific antibody development?

CUSTOMIZED
DISCOVERY
SCHEMES

CUSTOMIZED DISCOVERY SCHEMES

We can combine complementary antibody discovery platforms such as phage display with hyperimmune or naïve libraries, or B-cell cloning via HybriFree, or use fully synthetic phage display library for the discovery of unique panel of binders that can later be engineered into various bi- and multispecific antibody formats.

ENGINEERING
EXPERTISE

ENGINEERING EXPERTISE

Designing bispecific and multispecific antibodies involves multiple engineering steps to ensure molecules with precise multi-target binding specificities and affinities, while ensuring molecule stability, functionality and productivity profile, to name a few. This demands intricate molecular engineering skills and full understanding of structural biology to balance complexity and efficacy.

COMPREHENSIVE
CHARACTERIZATION

COMPREHENSIVE CHARACTERIZATION

We know that the diverse nature and multifunctionality of bispecific and multispecific antibodies demand a wide range of assays and methods for thorough validation, making the characterization process complex. It’s within our capacity to generate, produce and characterize hundreds of variants, from simple productivity characteristics to full binding kinetics.

Typical workflow for bispecific antibody development

One-stop-shop for bispecifics.

From antigen design and production to immunization, followed by discovery, engineering, optimization, production, and purification, we always deliver!

Affinity maturation and engineering

We can rationally assess different antibody projects to find right strategies towards generation of high affinity antibodies.

Our proprietary platform has undergone thorough testing and analysis, and when coupled with expert manual execution, has demonstrated outstanding effectiveness in enhancing antibody affinity and specificity.

Affinity maturation can be combined with antibody humanization.

Why choose Icosagen for affinity maturation?

RAPID
TIMELINES

RAPID TIMELINES

Affinity maturation takes 4-8 weeks, depending on using a rational strategy, or an unbiased strategy.

RATIONAL
ENGINEERING

RATIONAL ENGINEERING

Is key for the affinity maturation to succeed. We will design and generate libraries, assisted by the structure-based computational analysis containing soft-randomization in key positions to increase the chances to improve affinities.

DEVELOPABILITY
PARAMETERS

DEVELOPABILITY PARAMETERS

For therapeutic as well as any other purposes, the developability is crucial - we can humanize and engineer mAbs to improve their solubility, thermostability, in addition to affinity.

Affinity maturation and engineering workflow

Humanization of antibodies discovered from non-humanized animals or libraries is a necessity for their use as therapeutics. The challenge is to retain the desired specificity and affinity, while engineering away their immunogenic properties.

Antibody humanization

Our humanization platform service benefits from a proprietary software developed in collaboration with the University of Tartu.

We routinely humanize variable regions and framework sequences from any species, using any reference sequence as the basis. Key residues important for the VH/VL interface and antibody structure are maintained as much as possible in the humanized variants. Humanized sequences are screened to be free of motifs that are prone to glycosylation, deamidation or other PTMS.

FLEXIBILITY

FLEXIBILITY

Antibody variable regions from the light (VL) and heavy (VH) chains can be humanized from any species.

VALIDATED
SYSTEM

VALIDATED

In-house humanization and production cycle studies have provided antibodies with great titers and affinity database. We humanize all unconsolidated and non-human immunoglobulin variable region sequences.

FREE OF
SEQUENCE
LIABILITIES

FREE OF SEQUENCE LIABILITIES

Humanized sequences are screened to be free of motifs that can be prone to glycosylation or deamination.

KEY RESIDUES
MAINTAINED

KEY RESIDUES MAINTAINED

Key residues important for the VH/VL interface and structure are maintained as much as possible in the humanized variants.

DEVELOPABILITY

DEVELOPABILITY

Humanized antibodies show ideal developability profile compared to parental antibody.

Humanization workflow

Keep only the best binders with highest production yields

Let's discuss antibody development!

Antibody
discovery
Protein
production
Cell line
development
Analytics

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