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Our HybriFree Technology is based on B-cell cloning and used for efficient discovery of monoclonal antibodies from immunized chickens and rabbits, ready for their immediate production in mammalian cells. HybriFree antibody discovery is rapid, flexible and effective. It can also be used for cloning antibodies from recovered patients’ PBMCs or from existing hybridomas.
Recruit patients recovered from Covid-19
Isolate SARS-CoV-2 neutralizing antibodies
Identify lead candidate(s)
Manufacturing (GMP) Clinical trials
Total of 187 convalescent blood donors:
Positive PCR testMild to moderate symptomsSome hospitalized, but not in ICUCriteria for antibody cloning:
Tested ACE2 blocking efficacy and live virus neutralization potency of patient serumPatient with high viral neutralization potency were selected for antibody cloning
Modelling with two 23G7 Fabs bound to the tri-S protein indicated sterical interference of the Fabs limiting the unbound RBD to move in the "up" ACE2 binding conformation
1. Immunization of rabbits with the SARS-CoV-2 S1 protein (Wuhan)
2. Development of antibodies via HybriFree platform
3. Screening against VoC (Variant of concern) to identify cross-neutralizing clones
4. Sequence humanization
79C3 clone was identified as potent SARS-CoV-2 VoC neutralizing antibody. Humanization on 79C3 retained developability and functional neutralization profile.
We identified highly potent SARS-CoV-2 neutralizing antibodies that were active at low picomolar concentrations
We identified the structural characteristics of our lead 23G7 antibody molecule
23G7 antibody elicited effective virus neutralizing efficacy in Syrian Golden Hamster models
We identified VoC neutralizing antibodies
Formatting IgG into secretory IgA results in increased neutralization activity and resistance to independent mutations
Nebulized antibody delivery through inhalation results in broad respiratory tract distribution that leads to significantly reduced viral load in NHP models
ยฉ 2026 Icosagen
