Microbiolo­gical testing services

I Quantification of micro-organisms from food, feed (cfu/g, cfu/ml) including durability tests​

Analysis

Method

1 β-glucuronidase positive Escherichia coli

EVS-ISO
16649-2:2011+/AC:2017

2 Bacillus cereus

EVS-EN ISO
7932:2005

3 Bacillus spp.*

EVS-EN ISO
7932:2005, modified

4 Bacillus spp spores*

EVS-EN ISO
7932:2005, modified

5 Clostridium perfringens

EVS-EN ISO 15213-2:2023

6 Coliforms

ISO 4832:2006

7 Enterobacteriaceae

EVS-EN ISO 21528-2:2017

8 Coagulase-positive staphylococci (S. aureus etc)

EVS-EN ISO 6888-1:2021

9 Listeria monocytogenes

EVS-EN ISO 11290-2:2017

10 Total count of microorganisms

EVS-EN ISO
4833-1:2013+A1:2022

11 Mesophilic anaerobic microorganisms*

EVS-EN ISO
4833-1:2013+A1:2022, modified

12 Mesophilic aerobic spores*

EVS-EN ISO
4833-1:2013+A1:2022, modified

13 Mesophilic anaerobic spores*

EVS-EN ISO
4833-1:2013+A1:2022, modified

14 Mesophilic lactic acid bacteria

ISO 15214:1998

15 Yeasts and molds

EVS-ISO 21527-1:2009

EVS-ISO 21527-2:2009

16 Sulfite-Reducing Clostridium spp (clostridium)

ISO 15213:2023

17 Sterility control of canned goods**, includes  sensory analysis, microbiological analyses, and pH determination

NMKL 59 (5):2004

*Unaccredited method
**Complex analysis that includes both accredited and unaccredited methods

II Quantitative determination of microorganisms in surface samples

Analysis

Method

1 β-glucuronidase positive Escherichia coli

EVS-ISO
16649-2:2011/AC:2017

2 Coliforms

ISO 4832:2006

3 Enterobacteriaceae

EVS-EN ISO
21528-2:2017

4 Enterobacteriaceae (carcase sample)

EVS-EN ISO
21528-2:2017

5 Total count of microorganisms

EVS-EN ISO
4833-1:2013+A1:2022

6 Yeasts and molds*

EVS-ISO
21527-2:2009

*The methods for hygiene samples is unaccredited

III Detection of pathogens from food, surface samples, feed and primary production samples

Analysis

Method

1 Listeria monocytogenes (25 g)

EVS-EN ISO
11290-1:2017+/AC:2018

2 Listeria monocytogenes (25 g), PCR method

PCR meetod
JU-MB-49, JU-MB-50

3 Salmonella spp. (10g, 25g)

EVS-EN ISO
6579-1:2017+A1:2020

4 Salmonella spp, 50 g,
(100 g, 125 g, 250 g, 375 g, 750 g)

EVS-EN ISO
6579-1:2017+A1:2020

5 Surface sample – Listeria monocytogenes

EVS-EN ISO
11290-1+AC:2018

6 Surface sample – Listeria monocytogenes, molecular biology method (PCR)

PCR-meetod
JU-MB-49, JU-MB-50

7 Surface sample- Salmonella spp

EVS-EN ISO
6579-1:2017+A1:2020

8 Surface sample – Salmonella spp, molecular biology method (PCR)

PCR-meetod
JU-MB-43, JU-MB-44

9 Salmonella spp, primary production (carcase samples)

EVS-EN ISO
6579-1:2017+A1:2020

Sampling kits (sponges, swabs) are provided free of charge to Icosagen AS clients.

IV Chemical analysis of food samples

Analysis

Method

1 NaCl-Na

JU-MB-59

2 NaCl-Cl

JU-MB-73

2 Humidity

JU-MB-60

3 Food pH determination

ISO 2917; NMKL 179

V Microbiological analysis of water samples

Analysis

Method

1 Escherichia coli MPN method (Colilert-18)

EVS-EN ISO
9308-2:2014

2 Coliforms, MPN method (Colilert-18)

EVS-EN ISO
9308-2:2014

3 Intestinal Enterococci, MPN method (Enterlolert DW)

JU-MB-62

4 Enumeration of culturable microorganisms (22 °C)

EVS-EN ISO
6222:2001

5Enumeration of culturable microorganisms (37 °C)

EVS-EN ISO
6222:2001

6 Pseudomonas aeruginosa detection in 250 ml (Pseudalert)

JU-MB-70

7 Escherichia coli detection in 250ml 

JU-MB-72

8 Coliforms, detection in 250ml 

JU-MB-72

9 Intestinal enterococci, detection in 250ml 

JU-MB-71

10 Sulfite-reducing anaerobes with spores*

EVS-EN 26461-2:1999

11 Salmonella spp*

EVS-EN ISO 19250-2013

* Non-accredited method

VI Microbiological analyses of air samples

Analysis

Method

1Enumeration of total aerobic bacteria in air by the passive sedimentation method*

JU-MB-52

2 Quantitative determination of yeasts and molds in air using passive sedimentation method*

JU-MB-52

* Non-accredited method

VII Microbiological analyses of pharmaceutical products and active substances, preparations and formulations containing antibodies against viruses

Name

Method

1Total Aerobic Microbial Count (TAMC)

Ph.Eur 11; 2.6.12 JU-MB-67

2Total Yeast & Mold Count (TYMC)

Ph.Eur 11; 2.6.12 JU-MB-67

3Validation of the TAMC or TYMC method for the investigated matrix using a single CRM culture

Ph.Eur 11; 2.6.12 JU-MB-67

4 Escherichia coli detection

Ph.Eur 11; 2.6.13 JU-MB-69

5Validation of the Escherichia coli detection method for the investigated matrix using a single CRM culture

Ph.Eur 11; 2.6.13 JU-MB-69

6Sterility control with direct inoculation method

Ph.Eur 11; 2.6.1 JU-MB-68

5Validation of the sterility control method for the investigated matrix using a single CRM culture

Ph.Eur 11; 2.6.1 JU-MB-68

VIII Microbiological control of the production environment and water

Analysis

Method

1Determination of the total bacterial count in surface samples using the swabbing method

JU-MBF-009

2 Determination of yeasts and molds in surface samples using the swabbing method*

JU-MBF-009

3 Determination of the total bacterial count in air using the passive sedimentation method*

JU-MBF-006

4 Quantitative determination of yeasts and molds in air using the passive sedimentation method*

JU-MBF-006

5 Quantitative determination of cultivable microorganisms in WFI using the membrane filtration method*

JU-MBF-017

* Non-accredited method

Sampling kits (sponges, swabs, ready-to-use Petri dishes with agar medium) are provided free of charge to Icosagen AS clients.

IX Bacterial growth media (available on order)

Name

1 Dichloran glycerol agar (DG18)

2 Mannitol egg yolk polymyxin agar (MYP)

3 Plate count agar (PCA)

4 Sabouraud agar

5 Tryptone soya agar (TSA)

X Additional services

Analysis

Method

1 Autoclave performance check with biological indicator*

JU-MB-46

2 Identification of bacteria by 16S rRNA fragment sequencing* (partially otusourced)

JU-MB-58

3 Identification of bacteria with MALDI-TOF MS*

Outsourced

4 Microscopy*

JU-MB-21

5Quality control of microbiological growth media with CRM strains*

EVS-EN ISO
11133:2014+A1+A2:2020

6Mathematical modeling of the growth of Listeria monocytogenes*

FSSP model version 4.0

7 Collection of drinking water; laboratory certified sampler

EVS-EN ISO
19458:2006; JU-MB-41

8 Collection of hygiene samples*

EVS-EN ISO
18593:2018; JU-MB-35

9 Food sensory analysis*

JU-MB-42

10  Interlaboratory comparison*

11 Transport

*Non-accredited method

Consultation

• Consultation on general food microbiology
• Consultation on food hygiene for food preparation workers
• Consultation on molecular biology for microbiologists in the food industry
• PCR method as a tool for pathogen identification from food

With any questions or concerns please contact our Head of Laboratory Mrs Ene Talpsep by e-mail: ene.talpsep@icosagen.ee.

Latest news

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