Gildemann K, Tsernant ML, Liivand L, Ennomäe R, Poikalainen V, Lepasalu L, Rom S, Kavak A, Cox RM, Wolf JD, Lieber CM, Plemper RK, Männik A, Ustav M Jr, Ustav M, Gerhold JM. Anti-SARS-CoV-2 antibodies in a nasal spray efficiently block viral transmission between ferrets. iScience. 2024 Jun 20;27(7):110326. Read the paper here.
Guo X, Asthana P, Zhai L, Cheng KW, Gurung S, Huang J, Wu J, Zhang Y, Mahato AK, Saarma M, Ustav M, Kwan HY, Lyu A, Chan KM, Xu P, Bian ZX, Wong HLX. Artesunate treats obesity in male mice and non-human primates through GDF15/GFRAL signalling axis. Nat Commun. 2024 Feb 3;15(1):1034.
Tessier TM, Chowdhury A, Stekel Z, Fux J, Sartori MA, Teyra J, Jarvik N, Chung J, Kurinov I, Sicheri F, Sidhu SS, Singer AU, Zhang W. Structural and functional validation of a highly specific Smurf2 inhibitor. Protein Sci. 2024 Feb;33(2):e4885.
2023
Tolle I, Oehm S, Hoesl MG, Treiber-Kleinke C, Peil L, Bozukova M, Albers S, Adamu Bukari A-R, Semmler T, Rappsilber J, Ignatova Z, Gerstein AC and Budisa N. Evolving a mitigation of the stress response pathway to change the basic chemistry of life. Front. Synth. Biol. 2023 Aug 24; 1:1248065. Read the paper here.
Hermet P, Delache B, Herate C, Wolf E, Kivi G, et al. (2023) Broadly neutralizing humanized SARS-CoV-2 antibody binds to a conserved epitope on Spike and provides antiviral protection through inhalation-based delivery in non-human primates. PLOS Pathogens. 2023 Aug 2; 19(8): e1011532. Read the paper here.
Kovaleva V, Yu LY, Ivanova L, Shpironok O, Nam J, Eesmaa A, Kumpula EP, Sakson S, Toots U, Ustav M, Huiskonen JT, Voutilainen MH, Lindholm P, Karelson M, Saarma M. MANF regulates neuronal survival and UPR through its ER-located receptor IRE1α. Cell Rep. 2023 Feb 3;42(2):112066. Read the paper here.
2022
Porosk L, Nebogatova J, Härk HH, Vunk B, Arukuusk P, Toots U, Ustav M, Langel Ü, Kurrikoff K. Predicting Transiently Expressed Protein Yields: Comparison of Transfection Methods in CHO and HEK293. Pharmaceutics. 2022 Sep 14;14(9):1949. Read the paper here.
Naaber P, Tserel L, Kangro K, Punapart M, Sepp E, Jürjenson V, Kärner J, Haljasmägi L, Haljasorg U, Kuusk M, Sankovski E, Planken A, Ustav M, Žusinaite E, Gerhold JM, Kisand K, Peterson P. Protective antibodies and T cell responses to Omicron variant after the booster dose of BNT162b2 vaccine. Cell Rep Med. 2022 Aug 16;3(8):100716. Read the paper here.
Kangro K, Roose E, Dekimpe C, Vandenbulcke A, Graça NAG, Voorberg J, Ustav M, Männik A, Vanhoorelbeke K. Improvement of recombinant ADAMTS13 production through a more optimal signal peptide or an N-terminal fusion protein. J Thromb Haemost. 2022 Oct;20(10):2379-2385. Read the paper here.
Nacer A, Kivi G, Pert R, Juronen E, Holenya P, Aliprandini E, Amino R, Silvie O, Quinkert D, Le Duff Y, Hurley M, Reimer U, Tover A, Draper SJ, Gilbert S, Ho MM, Bowyer PW. Expanding the Malaria Antibody Toolkit: Development and Characterisation of Plasmodium falciparum RH5, CyRPA, and CSP Recombinant Human Monoclonal Antibodies. Front Cell Infect Microbiol. 2022 Jun 16;12:901253. Read the paper here.
Kangro K, Kurašin M, Gildemann K, Sankovski E, Žusinaite E, Lello LS, Pert R, Kavak A, Poikalainen V, Lepasalu L, Kuusk M, Pau R, Piiskop S, Rom S, Oltjer R, Tiirik K, Kogermann K, Plaas M, Tiirats T, Aasmäe B, Plaas M, Mumm K, Krinka D, Talpsep E, Kadaja M, Gerhold JM, Planken A, Tover A, Merits A, Männik A, Ustav M Jr, Ustav M. Bovine colostrum-derived antibodies against SARS-CoV-2 show great potential to serve as prophylactic agents. PLoS One. 2022 Jun 10;17(6):e0268806. Read the paper here.
Uusküla A, Keis A, Toompere K, Planken A, Rebrov K. Cluster randomised, controlled, triple-blind trial assessing the efficacy of intranasally administered virus-neutralising bovine colostrum supplement in preventing SARS-CoV-2 infection in household contacts of SARS-CoV-2-positive individuals: a study protocol. Trials. 2022 Jan 31;23(1):92. Read the paper here.
2021
Kuznetsov A, Arukuusk P, Härk H, Juronen E, Ustav M, Langel Ü, Järv J. ACE2 Peptide Fragment Interaction with Different S1 Protein Sites. Int J Pept Res Ther. 2022;28(1):7. Read the paper here.
Kasemaa K, Talts K, Täht E, Toots U, Ustav M. DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent. Methods Mol Biol. 2022;2383:569-578. Read the paper here.
De Waele L, Curie A, Kangro K, Tellier E, Kaplanski G, Männik A, Tersteeg C, Joly BS, Coppo P, Veyradier A, De Meyer SF, Roose E, Vanhoorelbeke K. Anti-cysteine/spacer antibodies that open ADAMTS13 are a common feature in iTTP. Blood Adv. 2021 Nov 9;5(21):4480-4484. Read the paper here.
Naaber P, Tserel L, Kangro K, Sepp E, Jürjenson V, Adamson A, Haljasmägi L, Rumm AP, Maruste R, Kärner J, Gerhold JM, Planken A, Ustav M, Kisand K, Peterson P. Dynamics of antibody response to BNT162b2 vaccine after six months: a longitudinal prospective study. Lancet Reg Health Eur. 2021 Nov;10:100208. Read the paper here.
Kangro K, Roose E, Joly BS, Sinkovits G, Falter T, von Auer C, Rossmann H, Reti M, Voorberg J, Prohászka Z, Lämmle B, Coppo P, Veyradier A, De Meyer SF, Männik A, Vanhoorelbeke K. Anti-ADAMTS13 autoantibody profiling in patients with immune-mediated thrombotic thrombocytopenic purpura. Blood Adv. 2021 Sep 14;5(17):3427-3435. Read the paper here.
Miersch S, Li Z, Saberianfar R, Ustav M, Brett Case J, Blazer L, Chen C, Ye W, Pavlenco A, Gorelik M, Garcia Perez J, Subramania S, Singh S, Ploder L, Ganaie S, Chen RE, Leung DW, Pandolfi PP, Novelli G, Matusali G, Colavita F, Capobianchi MR, Jain S, Gupta JB, Amarasinghe GK, Diamond MS, Rini J, Sidhu SS. Tetravalent SARS-CoV-2 Neutralizing Antibodies Show Enhanced Potency and Resistance to Escape Mutations. J Mol Biol. 2021 Sep 17;433(19):167177. Read the paper here.
Horta S, Neumann F, Yeh SH, Langseth CM, Kangro K, Breukers J, Madaboosi N, Geukens N, Vanhoorelbeke K, Nilsson M, Lammertyn J. Evaluation of Immuno-Rolling Circle Amplification for Multiplex Detection and Profiling of Antigen-Specific Antibody Isotypes. Anal Chem. 2021 Apr 20;93(15):6169-6177. Read the paper here.
Dekimpe C, Roose E, Kangro K, Bonnez Q, Vandenbulcke A, Tellier E, Kaplanski G, Feys HB, Tersteeg C, Männik A, De Meyer SF, Vanhoorelbeke K. Determination of anti-ADAMTS-13 autoantibody titers in ELISA: Influence of ADAMTS-13 presentation and autoantibody detection. J Thromb Haemost. 2021 Sep;19(9):2248-2255. Read the paper here.
Hrdinová J, D’Angelo S, Graça NAG, Ercig B, Vanhoorelbeke K, Veyradier A, Voorberg J, Coppo P. Dissecting the pathophysiology of immune thrombotic thrombocytopenic purpura: interplay between genes and environmental triggers. Haematologica. 2021 Mar 1;106(3):924. Read the paper here.
Ercig B, Graça NAG, Kangro K, Arfman T, Wichapong K, Hrdinová J, Kaijen P, van Alphen FPJ, van den Biggelaar M, Vanhoorelbeke K, Veyradier A, Coppo P, Reutelingsperger C, Nicolaes GAF, Männik A, Voorberg J. N-glycan-mediated shielding of ADAMTS13 prevents binding of pathogenic autoantibodies in immune-mediated TTP. Blood. 2021 May 13;137(19):2694-2698. Read the paper here.
2020
Velásquez Pereira LC, Roose E, Graça NAG, Sinkovits G, Kangro K, Joly BS, Tellier E, Kaplanski G, Falter T, Von Auer C, Rossmann H, Feys HB, Reti M, Prohászka Z, Lämmle B, Voorberg J, Coppo P, Veyradier A, De Meyer SF, Männik A, Vanhoorelbeke K. Immunogenic hotspots in the spacer domain of ADAMTS13 in immune-mediated thrombotic thrombocytopenic purpura. J Thromb Haemost. 2021 Feb;19(2):478-488. Read the paper here.
Graça NAG, Ercig B, Carolina Velásquez Pereira L, Kangro K, Kaijen P, Nicolaes GAF, Veyradier A, Coppo P, Vanhoorelbeke K, Männik A, Voorberg J. Modifying ADAMTS13 to modulate binding of pathogenic autoantibodies of patients with acquired thrombotic thrombocytopenic purpura. Haematologica. 2020 Nov 1;105(11):2619-2630. Read the paper here.
Ianevski A, Yao R, Biza S, Zusinaite E, Mannik A, Kivi G, Planken A, Kurg K, Tombak EM, Ustav M Jr, Shtaida N, Kulesskiy E, Jo E, Yang J, Lysvand H, Løseth K, Oksenych V, Aas PA, Tenson T, Vitkauskienė A, Windisch MP, Fenstad MH, Nordbø SA, Ustav M, Bjørås M, Kainov DE. Identification and Tracking of Antiviral Drug Combinations. Viruses. 2020 Oct 18;12(10):1178. Read the paper here.
Kangro K, Roose E, Schelpe AS, Tellier E, Kaplanski G, Voorberg J, De Meyer SF, Männik A, Vanhoorelbeke K. Generation and validation of small ADAMTS13 fragments for epitope mapping of anti-ADAMTS13 autoantibodies in immune-mediated thrombotic thrombocytopenic purpura. Res Pract Thromb Haemost. 2020 Jun 25;4(5):918-930. doi: 10.1002/rth2.12379. Read the paper here.
2019
Laaneväli A, Ustav M, Ustav E, Piirsoo M. E2 protein is the major determinant of specificity at the human papillomavirus origin of replication. PLoS One. 2019 Oct 23;14(10):e0224334. Read the paper here.
Pereira Aguilar P, Schneider TA, Wetter V, Maresch D, Ling WL, Tover A, Steppert P, Jungbauer A. Polymer-grafted chromatography media for the purification of enveloped virus-like particles, exemplified with HIV-1 gag VLP. Vaccine. 2019 Nov 8;37(47):7070-7080. Read the paper here.
Reiter K, Aguilar PP, Wetter V, Steppert P, Tover A, Jungbauer A. Separation of virus-like particles and extracellular vesicles by flow-through and heparin affinity chromatography. J Chromatogr A. 2019 Mar 15;1588:77-84. Read the paper here.
2018
Öhlund P, García-Arriaza J, Zusinaite E, Szurgot I, Männik A, Kraus A, Ustav M, Merits A, Esteban M, Liljeström P, Ljungberg K. DNA-launched RNA replicon vaccines induce potent anti-Ebolavirus immune responses that can be further improved by a recombinant MVA boost. Sci Rep. 2018 Aug 20;8(1):12459. Read the paper here.
Podgórska M, Ołdak M, Marthaler A, Fingerle A, Walch-Rückheim B, Lohse S, Müller CSL, Vogt T, Ustav M, Wnorowski A, Malejczyk M, Majewski S, Smola S. Chronic Inflammatory Microenvironment in Epidermodysplasia Verruciformis Skin Lesions: Role of the Synergism Between HPV8 E2 and C/EBPβ to Induce Pro-Inflammatory S100A8/A9 Proteins. Front Microbiol. 2018 Mar 7;9:392. Read the paper here.
Lázaro-Frías A, Gómez-Medina S, Sánchez-Sampedro L, Ljungberg K, Ustav M, Liljeström P, Muñoz-Fontela C, Esteban M, García-Arriaza J. Distinct Immunogenicity and Efficacy of Poxvirus-Based Vaccine Candidates against Ebola Virus Expressing GP and VP40 Proteins. J Virol. 2018 May 14;92(11):e00363-18. Read the paper here.
Sankovski E, Abroi A, Ustav M Jr, Ustav M. Nuclear myosin 1 associates with papillomavirus E2 regulatory protein and influences viral replication. Virology. 2018 Jan 15;514:142-155. Read the paper here.
2017
Toots M, Ustav M Jr, Männik A, Mumm K, Tämm K, Tamm T, Ustav E, Ustav M. Identification of several high-risk HPV inhibitors and drug targets with a novel high-throughput screening assay. PLoS Pathog. 2017 Feb 9;13(2):e1006168. Read the paper here.
Steppert P, Burgstaller D, Klausberger M, Tover A, Berger E, Jungbauer A. Quantification and characterization of virus-like particles by size-exclusion chromatography and nanoparticle tracking analysis. J Chromatogr A. 2017 Mar 3;1487:89-99. Read the paper here.
Steppert P, Burgstaller D, Klausberger M, Kramberger P, Tover A, Berger E, Nöbauer K, Razzazi-Fazeli E, Jungbauer A. Separation of HIV-1 gag virus-like particles from vesicular particles impurities by hydroxyl-functionalized monoliths. J Sep Sci. 2017 Feb;40(4):979-990. Read the paper here.
2016
Galli E, Härkönen T, Sainio MT, Ustav M, Toots U, Urtti A, Yliperttula M, Lindahl M, Knip M, Saarma M, Lindholm P. Increased circulating concentrations of mesencephalic astrocyte-derived neurotrophic factor in children with type 1 diabetes. Sci Rep. 2016 Jun 30;6:29058. Read the paper here.
Steppert P, Burgstaller D, Klausberger M, Berger E, Aguilar PP, Schneider TA, Kramberger P, Tover A, Nöbauer K, Razzazi-Fazeli E, Jungbauer A. Purification of HIV-1 gag virus-like particles and separation of other extracellular particles. J Chromatogr A. 2016 Jul 15;1455:93-101. Read the paper here.
Kivi G, Teesalu K, Parik J, Kontkar E, Ustav M Jr, Noodla L, Ustav M, Männik A. HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species. BMC Biotechnol. 2016 Jan 8;16:2. Read the paper here.
2015
Orav M, Geimanen J, Sepp EM, Henno L, Ustav E, Ustav M. Initial amplification of the HPV18 genome proceeds via two distinct replication mechanisms. Sci Rep. 2015 Nov 2;5:15952. Read the paper here.
Saare M, Hämarik U, Venta R, Panarina M, Zucchelli C, Pihlap M, Remm A, Kisand K, Toots U, Möll K, Salupere R, Musco G, Uibo R, Peterson P. SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis. J Immunol Res. 2015;2015:526518. Read the paper here.
Ustav M Jr, Castaneda FR, Reinson T, Männik A, Ustav M. Human Papillomavirus Type 18 cis-Elements Crucial for Segregation and Latency. PLoS One. 2015 Aug 19;10(8):e0135770. Read the paper here.
Karro K, Männik T, Männik A, Ustav M. DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent. Methods Mol Biol. 2015;1324:435-45. Read the paper here.
Reinson T, Henno L, Toots M, Ustav M Jr, Ustav M. The Cell Cycle Timing of Human Papillomavirus DNA Replication. PLoS One. 2015 Jul 1;10(7):e0131675. Read the paper here.
Isok-Paas H, Männik A, Ustav E, Ustav M. The transcription map of HPV11 in U2OS cells adequately reflects the initial and stable replication phases of the viral genome. Virol J. 2015 Apr 14;12:59. Read the paper here.
2014
Toots M, Männik A, Kivi G, Ustav M Jr, Ustav E, Ustav M. The transcription map of human papillomavirus type 18 during genome replication in U2OS cells. PLoS One. 2014 Dec 30;9(12):e116151. Read the paper here.
dos Santos R, Rosa SA, Aires-Barros MR, Tover A, Azevedo AM. Phenylboronic acid as a multi-modal ligand for the capture of monoclonal antibodies: development and optimization of a washing step. J Chromatogr A. 2014 Aug 15;1355:115-24. Read the paper here.
