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Recombinant antibody HCV 1b NS5B (clone R9A2)

Recombinant antibody HCV 1b NS5B (clone R9A2)

Figure 1. Western Blot testing of anti-HCV NS5B protein using chimeric anti-HCV NS5B antibody 9A2. Line 1. Protein size marker,  Line 2. 20 ng of purified recombinant HCV NS5B was loaded per lane.

 
Recombinant antibody HCV 1b NS5B (clone R9A2)

Figure 2. Immunofluorescence analysis on ETAR protein fused with 9A2 epitope tag. ETAR-9A2 is expressed in CHOEBNALT85 cell line.

Select volume:

Quantity:

€ 2307

Catalogue #

C2-424-100

Name

Recombinant antibody HCV 1b NS5B (clone R9A2)

Target

HCV 1b NS5B

Target Description

Recombinant Hepatitis C Virus subtype 1b non-structural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)

Uniprot ID

Q9WMX2

Clonality

Human monoclonal

Clone

R9A2

Class

hIgG1

Reactivity

Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT)

Application

ELISA, WB, IF

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

ELISA:

5 - 10 µg/ml

WB:

1 µg/ml under reducing conditions.

IF:

dilution 1:1000

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Related Products

This antibody is derived from A2-424-100 mouse monoclonal antibody to HCV 1b NS5B, clone 9A2. HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/products/?antibodies

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Background

Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. Antibody is derived from mouse anti-HCV NS5B monoclonal antibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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