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Partially humanized, chimeric recombinant monoclonal antibody to HIV-1 p24 (clone 5)

Figure 1. Western-Blot analysis of HIV-1 HAN subtype p17/24 protein using chimeric HIV-1 p24 antibody, clone 5. Line 1. Protein size marker Line 2. HIV-1 p17/24 20 ng per lane.

     

Figure 2. IF analysis using chimeric HIV-1 p24 antibody, clone 5. ETAR (Endothelin A receptor) is fused with 05-001 epitope tag, expressed in  CHOEBNALT85 cell line and detected in IF assay.

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Quantity:

€ 2098

Catalogue #

C-851-100

Name

Partially humanized, chimeric recombinant monoclonal antibody to HIV-1 p24 (clone 5)

Target

HIV-1 p24

Target Description

Recombinant p24 protein of HIV-1 HAN consensus (purified from E. coli)

Clonality

Human monoclonal

Clone

5

Class

hIgG1

Reactivity

Reacts with HIV-1 p24 subtype B, C and A. Mapped to 48-62(TPQDLNTMLNTVGGH)

Application

ELISA, WB

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

For detection please use anti-human IgG antibody

ELISA:

0.16 to 0.3 µg/ml

WB:

1 µg/ml

IF:

1 to 10 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, 50% glycerole

Related Products

Mouse anti-HIV 1 p24 monoclonal antibody clone 5 A2-851-100. HIV-1 p24 monoclonal antibodies.

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

Background

Antibody is derived from mouse anti-HIV-1 (HAN subtype) p17/24 monoclonal antoibody clone 5 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 05-001 epitope-tag sequence.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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