Otsi
Otsi
Figure 1. Western-Blot analysis of HIV-1 HAN subtype p17/24 protein using chimeric HIV-1 p24 antibody, clone 5. Line 1. Protein size marker Line 2. HIV-1 p17/24 20 ng per lane.
Figure 2. IF analysis using chimeric HIV-1 p24 antibody, clone 5. ETAR (Endothelin A receptor) is fused with 05-001 epitope tag, expressed in CHOEBNALT85 cell line and detected in IF assay.
Catalogue #
C-851-100
Name
Partially humanized, chimeric recombinant monoclonal antibody to HIV-1 p24 (clone 5)
Target
HIV-1 p24
Target Description
Recombinant p24 protein of HIV-1 HAN consensus (purified from E. coli)
Clonality
Human monoclonal
Clone
5
Class
hIgG1
Reactivity
Reacts with HIV-1 p24 subtype B, C and A. Mapped to 48-62(TPQDLNTMLNTVGGH)
Application
ELISA, WB
Protocol
ELISA:
0.16 to 0.3 µg/ml
WB:
1 µg/ml
IF:
1 to 10 µg/ml
Purification
Protein G purification
Buffer
PBS pH 7.4, 50% glycerole
Related Products
Mouse anti-HIV 1 p24 monoclonal antibody clone 5 A2-851-100. HIV-1 p24 monoclonal antibodies.
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background
Antibody is derived from mouse anti-HIV-1 (HAN subtype) p17/24 monoclonal antoibody clone 5 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 05-001 epitope-tag sequence.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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