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Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-COV-2 S1 UK VOC 202012/01 . 4-15% gradient gel is used for analysis. Lane 1. 1.3 µg SARS-CoV-2 Spike S1 UK VOC 202012/01 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg SARS-CoV-2 Spike S1 UK VOC 202012/01 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis of SARS-CoV-2 S1 protein (wild type, red line) and S1 UK variant (blue line) binding to the ACE2 receptor.
Catalogue #
P-310-100
Name
SARS-CoV-2 Spike S1 UK VOC 202012/01(alpha)
Description
cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1)
Protein contains amino acids 14-681, mutations HV 69-70 del, Y144 del, N501Y, A570D, P681H, D614G, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
MW
75.82 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Purification
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity
>95%
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
Bioproperties
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
QC
SDS-PAGE
NanoDrop A280, analytical SEC, Octet binding to ACE2 receptor
Shipping
Shipped on dry ice
Storage
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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