Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-COV-2 S1 UK VOC 202012/01 . 4-15% gradient gel is used for analysis. Lane 1. 1.3 µg SARS-CoV-2 Spike S1 UK VOC 202012/01 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg SARS-CoV-2 Spike S1 UK VOC 202012/01 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis of SARS-CoV-2 S1 protein (wild type, red line) and S1 UK variant (blue line) binding to the ACE2 receptor.
SARS-CoV-2 Spike S1 UK VOC 202012/01(alpha)
cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1)
Protein contains amino acids 14-681, mutations HV 69-70 del, Y144 del, N501Y, A570D, P681H, D614G, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
CHO-based cell line (expressed by QMCF Technology)
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
PBS pH 7.4
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
NanoDrop A280, analytical SEC, Octet binding to ACE2 receptor
Shipped on dry ice
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
This product is for research use only
Please refer any technical questions firstname.lastname@example.org