Mouse mAb to HIV-1 Tat (clone 1)

Catalogue #

A2-821-100

Name

Mouse mAb to HIV-1 Tat (clone 1)

Target

HIV-1 Tat

Target Description

Recombinant Tat protein of HIV-1 HXB2 (purified from E. coli)

Clonality

Mouse monoclonal

Clone

1

Class

mIgG2a

Reactivity

Reacts with HIV-1 Tat of HXB2, IIIB, HAN strains. This antibody recognizes Tat protein consensus sequences of HIV-1 subtypes A(DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE). Mapped to amino acids 2-9 EPVDPRLE ­-B subtype a.a. consensus

Application

ELISA, WB, IHC, RIP, ChIP

Protocol

ELISA:

0,02-0,05 µg/ml

WB:

1-2 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

References

Ranki A., A. Lagerstedt, V. Ovod, E. Aavik, and K. Krohn 1994.
Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef and Tat in established lymphoid cell lines. Arch. Virol., 139:365-378.

Ranki A., M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn 1995.
Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Tähtinen, M., Ranki, A., Valle,S.L., Ovod, V., K. Krohn 1997.
B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother 51: 480-7.

Henderson WW, Ruhl R, Lewis P, Bentley M, Nelson JA and Moses AV. 2004.
Human immunodeficiency virus (HIV) type 1 Vpu induces the expression of CD40 in endothelial cells and regulates HIV-induced adhesion of B-lymphoma cells. J Virol 78: 4408-20.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.