Mouse mAb to HIV-1 Tat (clone 7)

Catalogue #

A2-822-100

Name

Mouse mAb to HIV-1 Tat (clone 7)

Target

HIV-1 Tat

Target Description

Recombinant Tat protein of HIV-1 BH10 (purified from E. coli)

Clonality

Mouse monoclonal

Clone

7

Class

mIgG1

Reactivity

Reacts in ELISA, WB with HIV-1 Tat of BH10, IIIB strains. This antibody reacts with the rTat consensus sequences of HIV-1subtypes A (PNLEPWH), B (PRLEPWK) and C (PNLEPWN). Mapped to amino acids 6-12(PRLEPWK - B subtype a.a. consensus)

Application

ELISA, WB, ChIP

Protocol

ELISA:

0,05-0,1 µg/ml

WB:

1-2 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

References

Reid W., Sadowska M., Denaro, F. Rao S., Foulke J., Hayes N., Jones 0., Davis H., Sill A., O'Driscoll P., Huso, D., Fouts T., Lewis, G., Kames-Lowis R., Wei C., Ray P, Gallo, R., Reits, M., and Bryant J. 2001.
An HIV -1 Transgenic Rat that Develops HIV -1 related Pathology and Immunological Dysfunction. Proceedings of the National Academy of Science, 98 (16): 9271-9276.