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Mouse mAb to HIV-1 Tat (clone 7)

Figure 1. Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone 7). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml. Lane 2. Protein size marker.
 
Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone 7). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA.

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Quantity:

€ 2098

Catalogue #

A2-822-100

Name

Mouse mAb to HIV-1 Tat (clone 7)

Target

HIV-1 Tat

Target Description

Recombinant Tat protein of HIV-1 BH10 (purified from E. coli)

Clonality

Mouse monoclonal

Clone

7

Class

mIgG1

Reactivity

Reacts in ELISA, WB with HIV-1 Tat of BH10, IIIB strains. This antibody reacts with the rTat consensus sequences of HIV-1subtypes A (PNLEPWH), B (PRLEPWK) and C (PNLEPWN). Mapped to amino acids 6-12(PRLEPWK - B subtype a.a. consensus)

Application

ELISA, WB, ChIP

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

ELISA:

0,05-0,1 µg/ml

WB:

1-2 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

References

Reid W., Sadowska M., Denaro, F. Rao S., Foulke J., Hayes N., Jones 0., Davis H., Sill A., O'Driscoll P., Huso, D., Fouts T., Lewis, G., Kames-Lowis R., Wei C., Ray P, Gallo, R., Reits, M., and Bryant J. 2001.
An HIV -1 Transgenic Rat that Develops HIV -1 related Pathology and Immunological Dysfunction. Proceedings of the National Academy of Science, 98 (16): 9271-9276.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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