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Figure 1. Simply Blue Safe stained SDS-PAGE analysis of mAb to Hexahistidine tag, clone 24H10. 4-15% gradient gel is used for analysis. Lane 1. Monoclonal antibody to Hexahistidine tag, clone 24H10 (-DTT) Lane 2. Size marker. Lane 3. Monoclonal antibody to Hexahistidine tag, clone 24H10 (+DTT)
Figure 2. Analytical HPLC-SEC for final antibody product
Figure 3. Analytical HPLC-SEC for final antibody product after 3 freeze-thaw cyclesCatalogue #
A2-502-100
Name
Mouse mAb to Hexahistidine (His6) tag (clone 24H10)
Target
His-tag
Target Description
Recombinant C-terminal His6-tagged protein produced in E. coli
Clone
24H10
Class
hIgG1
Application
ELISA, WB
ELISA:
0.16 - 0.31 µg/ml
WB:
0.5 - 2 µg/ml
Purification
Protein A affinity chromatography following gel filtration
Buffer
PBS pH 7.4, 0.1% sodium azide
Shipping
This product is shipped in non-frozen liquid form in ambient conditions
Storage
The product is stable at 4 °C for several days. Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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