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Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 27C2)

Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27C2. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27C2 (-DTT). Lane 2. Size Marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27C2 (+DTT). Lane 4. Size Marker.

  Figure 2. HPLC analytical SEC for final product.  

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

          Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture of Spike S1 protein.

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Catalogue #

R1-158-100

Name

Human IgG1-lambda antibody to SARS CoV-2 S1 RBD (clone 27C2)

Target

SARS-CoV-2 virus

Target Description

SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology)

Clonality

Human monoclonal

Clone

27C2

Class

hIgG1

Reactivity

SARS-CoV-2 Spike RBD

Dissociation constant (KD)

< 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)

6.58 x 10-9 M (measured against SARS-CoV-2 Spike S1 protein)

Application

ELISA

ELISA:

0,02-0,04 ng/ml

Purification

Protein A affinity chromatography following desalting

Buffer

PBS pH 7.4

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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