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Figure 1. Simply Blue Safe Stain SDS-PAGE analysis of monoclonal antibody to Influenza B, clone 20B2. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg monoclonal antibody to Influenza B, clone 20B2 (-DTT). Lane 2. Size marker. Lane 3. 0.8 µg monoclonal antibody to Influenza B, clone 20B2 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture Flu b nucleocapsid protein.
Catalogue #
R1-204-100
Name
mAb to Influenza B, clone 20B2
Target
Recombinant Influenza B Nucleocapsid protein
Target Description
Recombinant Influenza B Nucleocapsid protein
Source
Human
Clonality
Human monoclonal
Clone
20B2
Class
hIgG1
Reactivity
Influenza B nucleocapsid protein, Influenza B Florida inactivated virus, Influenza B Malaisia inactivated virus, no cross-reactivity with Flu A nucleocapsid protein
Dissociation constant (KD)
<1.0 x 10-12 M (measured against Influenza B nucleocapsid protein)
Application
ELISA
Protocol
Purification
Protein A affinity chromatography following gel filtration.
Buffer
PBS pH 7.4
QC
Simply Blue Safe stained SDS-PAGE, analytical SEC, Octet binding
Shipping
This product is shipped with blue ice.
Storage
Store at +4 °C. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com