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Chicken pAb to human GFRa-3

 

Figure 1. IF detection of human GFRα-3 expressed in U2OS cells. GFRα-3 was visualized using Chicken polyclonal antibody to human GFRα-3 (dilution 1:3000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-3 specific signal and blue for nuclei.

                       

Figure 2. Western-Blot detection of human GFRα-3, using Chicken polyclonal antibody to human GFRα-3. GFRα-3 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-3. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody (Icosagen)  was used as secondary antibody.

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Quantity:

€ 2098

Catalogue #

305-100

Name

Chicken pAb to human GFRa-3

Target

human GFRa-3

Target Description

Recombinant His-tagged human GFRa-3 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant

Alternative Names

GDNF receptor alpha-3

Uniprot ID

O60609

Clonality

Polyclonal

Class

IgY

Reactivity

Human GFRa-3
Doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-4

Application

ELISA, WB, IF

Protocol

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

3-6,25 ng/ml

WB:

1-2 µg/ml

IF:

0,3-0,67 µg/ml

Purification

GFRα-3 affinity purified

Buffer

Concentrated ammonium sulphate in PBS pH 7.4

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use

Background

The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signalling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com
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