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Figure A. Coomassie-stained SDS-PAGE and Western Blot analysis on purified recombinant human Artemin. (A) SDS-PAGE analysis in reduced and non-reduced conditions. 2µg of recombinant human Artemin was loaded per lane. Lane 1 Artemin (reduced); Lane 2. Protein size marker (Blue Protein Ladder Prestained, Naxo, 8003); Lane 3. Artemin (non-reduced);
Figure B. Western Blot analysis of recombinant human Artemin. Anti-human Artemin monoclonal antibody clone 2D12 (Icosagen AS Cat. No 324-100) was used for testing. 2D12 antibody recognizes only native conformation, dimeric (non-reduced) Artemin. 0.2 µg of recombinant Artemin were loaded per lane. Lane 1. Artemin (reduced); Lane 2. Protein size marker Blue (Protein Ladder Prestained, Naxo, 8003); Lane 3. Artemin (non-reduced).
Catalogue #
P-102-100
Name
Artemin, human recombinant
Synonyms
Artemin, Enovin, Neublastin
Uniprot ID
Q5T4W7
Source
Human
MW
Approximately 18.8 kDa, a single nonglycosylated polypeptide chain containing 181 amino acids. Protein is homodimer
Host
CHO-based cell line (expressed by QMCF Technology)
Purification
Purified from serum-free CHO growth media by ion-exchange chromatography and gel-filtration. Protein is sterile-filtered through 0.22 µm filter
Concentration
1 mg/ml
Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
Buffer
10 mM Na-citrate; 150 mM NaCl pH 4.5
Endotoxine
Less than 1 EU/mg of protein as determined by LAL method
Bioproperties
Product is suitable for cell culture and in vivotesting. Protein is biologically active. Biological activity of purified artemin was tested in in vitroreceptor-dependent cellular morphology change induction assay using U2OS cells expressing Ret and GFRα-3 receptor complex
QC
SDS-PAGE and Western-Blot analysis
Related Products
Polyclonal and monoclonal antibodies against human Artemin
Shipping
Shipped in dry-ice
Storage
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
Background
Artemin is a ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut associated lymphoid tissue (www.uniprot.org; Baloh et al. 1998, Neuron; Rosenblad et al. 2000, Eur. J. Biochem)
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