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Rabbit polyclonal antibody to MLV-Gag protein

Rabbit polyclonal antibody to MLV-Gag protein                

Figure 1. Western Blot testing of MLV-gag antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas); Line 2. HEK293 cells; Line 3. MLV-gag protein expressed in HEK293 cells; Line 4. PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas); Line 5. CHO cells; Line 6. MLV-gag protein expressed in CHO cells. 10 000 cells were lysed and loaded into each lane.

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Quantity:

€ 2307

Catalogue #

A1-910-100

Name

Rabbit polyclonal antibody to MLV-Gag protein

Target

MLV p30

Target Description

Full length recombinant MLV-Gag p30 capside protein, isolate FB29, purified from E. coli

Clonality

Polyclonal

Class

IgG

Reactivity

MLV Gag, p30

Application

ELISA, WB

Protocol

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

ELISA:

0,01 µg/ml

WB:

0,5-1 µg/ml

Purification

MLV p30 affinity purified

Buffer

Ammonium sulphate, saturated (PBS pH 7.4)

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at +4°C upon receipt. As product is (NH4)2SO4precipitate, mix well by pipetting or vortexing prior use

Background

Murine leukemia viruses belong to family of Retroviruses group and are named for their ability to cause cancer in mice. Some form of MLV’s are used in cancer research. The retroviral Gag protein plays important roles at several stages in the viral life cycle. Late in infection, the Gag precursor protein acts to mediate the assembly and release of virion particles. After budding, the core matures by cleavage of Gag precursor protein into the mature core proteins, the matrix (MA), capsid (CA) and nucleocapsid proteins. These separated Gag proteins then play roles in the early stages of infection

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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