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SARS-CoV-2 Spike S1 with D614G

 

Figure 1.  Coomassie-stained SDS-PAGE analysis of SARS-CoV-2 Spike S1 with D614G. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg SARS-CoV-2 Spike S1 with D614G (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Spike S1 with D614G (+DTT).

       

Figure 2. HPLC analytical SEC for final product.

       

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

         

Figure 4.  Binding analysis of the SARS-CoV-2 Spike S1 with D614G protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.

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Quantity:

€ 1650

Catalogue #

P-321-100

Name

SARS-CoV-2 Spike S1 with D614G

Description

Protein contains amino acids 14-681, mutation D614G, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.

MW

75.82 kDa

Host

CHO-based cell line (expressed by QMCF Technology)

Kd:

3.72E-08 (measured against ACE2 receptor)

Purification

Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.

Purity

>95%

Concentration

1 mg/ml

Buffer

PBS pH 7.4

Endotoxine

NA

Bioproperties

Measured by its binding ability to ACE2 protein by OCTET RED96 system.

QC

SDS-PAGE
NanoDrop A280, analytical SEC, Octet binding to ACE2 receptor

Shipping

Shipped on dry ice

Storage

Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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