Mouse mAb to HIV-1 Nef (clone N5)

Catalogue #

A2-811-100

Name

Mouse mAb to HIV-1 Nef (clone N5)

Target

HIV-1 Nef

Target Description

Recombinant Nef protein of HIV-1 B-subtype consensus (purified from E. coli)

Alternative Names

3’ORF

Clonality

Mouse monoclonal

Clone

N5

Class

mIgG1

Reactivity

This antibody recognizes HIV-1 subtypes A, B and C. Mapped to amino acids 62-68 (EEEVGFP – B subtype consensus)

Application

ELISA, WB

Protocol

ELISA:

0,02-1 µg/ml

WB:

0,2 µg/ml

Purification

Protein G purification

Buffer

PBS pH 7.4, with 0.1% sodium azide

Related Products

HIV-1 Nef monoclonal antibodies. For more information visit www.icosagen.com/products/?antibodies

Shipping

This product is shipped in non-frozen liquid form in ambient conditions

Storage

Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

References

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999.
Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5-6):460-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001.
Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.