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mAb to Influenza A/B, clone 25F4

mAb to Influenza A/B, clone 25F4  

Figure 1. Simply Blue Safe Stain SDS-PAGE analysis of monoclonal antibody to Influenza A/B, clone 25F4. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg monoclonal antibody to Influenza A/B, clone 25F4 (-DTT). Lane 2. Size marker. Lane 3. 0.8 µg monoclonal antibody to Influenza A/B, clone 25F4 (+DTT).

      mAb to Influenza A/B, clone 25F4  

Figure 2. HPLC analytical SEC for final product.

      mAb to Influenza A/B, clone 25F4  

Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.

      mAb to Influenza A/B, clone 25F4  

Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture Flu b nucleocapsid protein.

                 

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Catalogue #

R1-209-100

Name

mAb to Influenza A/B, clone 25F4

Target

Recombinant Influenza B Nucleocapsid protein

Target Description

Recombinant Influenza B Nucleocapsid protein

Source

Human

Clonality

Human monoclonal

Clone

25F4

Class

hIgG1

Reactivity

Influenza B nucleocapsid protein, Influenza B Florida inactivated virus, Influenza B Malaisia inactivated virus. Show some cross-reactivity towards FluA nucleoprotein at higher antibody concentration.

Dissociation constant (KD)

<1.0 x 10-12 M (measured against Influenza B nucleocapsid protein)

Application

ELISA

Protocol

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification

Protein A affinity chromatography following gel filtration.

Buffer

PBS pH 7.4

Shipping

This product is shipped with blue ice.

Storage

Store at +4 °C. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to

technical.support@icosagen.com

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