Otsi
Otsi
Figure 1. Simply Blue Safe Stain SDS-PAGE analysis of monoclonal antibody to Influenza A/B, clone 25F4. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg monoclonal antibody to Influenza A/B, clone 25F4 (-DTT). Lane 2. Size marker. Lane 3. 0.8 µg monoclonal antibody to Influenza A/B, clone 25F4 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis, antibody was loaded on sensor for capture Flu b nucleocapsid protein.
Catalogue #
R1-209-100
Name
mAb to Influenza A/B, clone 25F4
Target
Recombinant Influenza B Nucleocapsid protein
Target Description
Recombinant Influenza B Nucleocapsid protein
Source
Human
Clonality
Human monoclonal
Clone
25F4
Class
hIgG1
Reactivity
Influenza B nucleocapsid protein, Influenza B Florida inactivated virus, Influenza B Malaisia inactivated virus. Show some cross-reactivity towards FluA nucleoprotein at higher antibody concentration.
Dissociation constant (KD)
<1.0 x 10-12 M (measured against Influenza B nucleocapsid protein)
Application
ELISA
Protocol
Purification
Protein A affinity chromatography following gel filtration.
Buffer
PBS pH 7.4
Shipping
This product is shipped with blue ice.
Storage
Store at +4 °C. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com