Peak #
RT (min)
Estimated Mw (Da)*
1
7.434
350364
2
8.907
132648
Area
Area %
21.11
1.03
2020.68
98.97
Chromatogram
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Time [min]
0
10
20
30
40
50
60
70
80
90
100
110
120
130
140
mAU
7.434
8.907
ICO230227 ICO0280-A01_pur_prod_final_1 3x, DAD 220 nm
*Calculated using calibration curve obtained from AdvanceBio SEC 300A Protein Standard (p/n 5190-9417) retention times.
Peaks with integrated areas below 0.5% of the calculated values were excluded from analysis.