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Icosagen has a dedicated department for protein purification & analytics to ensure meticulous product characterization for optimal quality of both antibodies and recombinant proteins.
For customized solutions we have a comprehensive list of analytical options available to cover all the needs for proteomic characterization.
Involves standard methods to analyze molecular weight, check molecule expression and secretion during production as well as confirm that produced antibodies are functional and bind to the target antigen.
Analytics plays a key role in protein production in order to get functional proteins with high purity. Most commonly used methods are:
Coomassie stained SDS-PAGE and LabChip capillary electrophoresis — a standard test to analyze:
molecular weight
check molecule expression and secretion during production
estimate product purity during purification.
Western Blot is used to detect proteins from different samples.
ELISA test:
confirm that produced recombinant proteins are functional
determine EC50 values for antibodies
perform competitive binding assays.
Analytical Size-Exclusion Chromatography (analytical HPLC-SEC):
characterize final product or purified fraction by molecular weight to monitor the purity of the product and detect the presence of aggregates or low molecular weight contaminants.
Analytical Ion-Exchange chromatography (analytical HPLC-IEX):
determine protein charge variant distribution
control final fraction of bispecific antibodies to assure no monospecific antibody versions are present, in case there is sufficient charge difference between these two.
This is usually supplemented with mass-spectrometry.
LAL-test (Charles River Endosafe® nexgen-PTS™):
determine the level of bacterial endotoxins in purified recombinant protein samples.
cGE by LabChip GXII Protein Characterization System (PerkinElmer) — provides the complete solution for product quantitation and molecular weight sizing or impurity and homogeneity analysis.
For customized solutions we have a comprehensive list of analytical options for glycosylation pattern analysis, stability studies in different stress conditions as well as formulation studies.
All-in-one protein stability analysis with the Uncle platform (Unchained Labs) — a stability platform that uses scanning fluorimetry (nanoDSF), static light scattering (SLS) and dynamic light scattering (DLS) detection methods to characterize protein stability. Allows to:
Biolayer interferometry (BLI) binding analysis — binding and affinity analysis with Octet platform (Sartorius):
Chemiluminescence immunoassay (CLIA):
Discover CLIA service information here.
Lunatic (Unchained Labs) – high-throughput concentration measurements with UV absorbance.
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