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Antibody development

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We help to tell your story with proteins

Offering custom-tailored services for antibody development, recombinant protein production as well as off-the-shelf-products, we provide full service from antigen production to protein-of-interest purification.


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Proteins produced annually
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Different proteins purified annually

Antibody discovery with HybriFree

HybriFree Technology is based on B-cell cloning, that can be used for efficient discovery of monoclonal antibodies and their immediate production in mammalian cells.
HybriFree makes antibody discovery rapid and flexible that can be even used for cloning antibodies from recovered patient’s PBMCs and existing hybridomas.


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Why choose HybriFree for antibody discovery?

FAST in vivo
APPROACH

FAST in vivo APPROACH

After immunization stage, biologically active antibodies with optimal physicochemical properties can be delivered as fast as 8-9 weeks

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EASY TO
PRODUCE

READY FOR DOWNSTREAM DEVELOPMENT

Of chickens can be used to develop antibodies against hard-to-express proteins

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IMMUNIZATION
WITH COMPLEX
ANTIGENS

DNA IMMUNIZATION

From peptides to VLPs, immunization of chickens can be used to develop antibodies against hard-to-express proteins

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FLEXIBLE

FLEXIBLE

Identified sequences can be used for production of different immune molecules (chimeras, antibody fragmens etc.)

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NO TARGET
IS DIFFICULT

NO TARGET IS DIFFICULT

Combination with Icosagen’s high yield QMCF mammalian expression system can be used to produce milligram to gram quantities of developed antigens used for immunization, including peptides, VLPs and difficult-to-express proteins

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HybriFree workflow

HybriFree is Icosagen’s proprietary technology, based on B-cell cloning, that can be used for efficient discovery of monoclonal antibodies and their immediate production in mammalian cells. It’s rapid and flexible method that can  be used for cloning antibodies from different species such as chicken and rabbits, recovered patient’s PBMCs and existing hybridomas (HybriRescue).

Options for immunization platform

chicken_block

Chicken

Fastest immunization

High affinity

DNA immunization

The best for conserved targets

Rabbit

Fast immunization

Highest affinity

Great diversity

The best for small molecules

mouse_block

Mouse

Slow immunization

Normal affinity

Industry standard


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Hybridomas are not frequently monospecific and express non functional pseudo-variables

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percent from 185 analyzed hybridomas were found to contain only one productive VH and VL gene

Hybridoma sequencing platform HybriRescue

We clone antibodies from hybridomas, provided by our customers, and produce them at requested scales.
In addition, we deliver the cDNA sequence encoding the antibody variable heavy (VH) and light (LH) domains.

ANTIBODY
ENGINEERING

DELIVERS ANTIBODY SEQUENCE

Essential for monoclonal antibody engineering, humanization, function optimization or patent applications

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PROVIDES
SECURITY

PROVIDES SECURITY

With determined antibody sequence there is always opportunity to reproduce it, which eliminates the risk of excellent antibodies being permanently lost due to loss of cell culture

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PATENT
APPLICATION

PATENT APPLICATION

Patent the sequences of your unique variable domains that provides opportunities for investing in R&D

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HybriRescue workflow

High affinity and specific therapeutic monoclonal antibodies that are comparable to native human antibodies to minimize their immunogenicity

Antibody humanization

Our humanization platform service is performed with proprietary program developed in collaboration with Icosagen and University of Tartu.

We can humanize variable region framework sequences from any species, using any reference. By taking advantage of HybriFree technology that doesn’t need hybridomas, the spleen B-cells are collected and VH and VL cDNA sequence is amplified.

Customer benefits

FLEXIBILITY

FLEXIBILITY

Antibody variable regions from the light (VL) and heavy (VH) chains can be humanized from any species

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VALIDATED
SYSTEM

VALIDATED

In-house humanization and production cycle studies have provided antibodies with great titers and affinity database. We humanize all unconsolidated and non-human immunoglobulin variable region sequences

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FREE OF MOTIFS

FREE OF MOTIFS

Humanized sequences are screened to be free of motifs that can be prone to glycosylation or deamination

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KEY RESIDUES
MAINTAINED

KEY RESIDUES MAINTAINED

Key residues important for the VH/VL interface and structure are maintained as much as possible in the humanized variants

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FLEXIBILITY

FLEXIBILITY

Antibody variable regions from the light (VL) and heavy (VH) chains can be humanized from any species

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Humanization workflow

For inquiries about antibody development please contact Luka Bulatovic

E-mail: luka.bulatovic@icosagen.ee

Phone: 

Follow up services


PROTEIN PRODUCTION


GMP COMPATIBLE CELL LINE DEVELOPMENT

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Our locations

United States Office

77 Geary St. 5th Floor
San Francisco, CA 94108
+1 415 548 6625

European Office

Eerika tee 1
Õssu, Kambja vald
Tartu maakond 61713
Estonia
+372 737 7070

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