List of services

Microbiological testing services

I Enumeration of microorganisms (cfu/g, cfu/ml) in food, feed and hygiene samples

Analysis
Method
1.
Total plate count
EVS-EN ISO 4833:2006
2.
Enterobacteriaceae
ISO 21528-2:2004
3.
β-glucuronidase positive Escherichia coli
ISO 16649-2:2001
4.
Coliforms
ISO 4832:2006
5.
Coagulase-positive staphylococci (S. aureus etc)
EVS-EN ISO 6888-1 2001/A1:2004
6.
Bacillus cereus
EVS-EN ISO 7932:2005
7.
Yeasts and molds
EVS ISO 21527- 1/2:2009
8.
Clostridium perfringens
EVS-EN ISO 7937:2004
9.
Listeria monocytogenes
EVS-EN ISO 11290- 2:2000/A1:2004
9.
Mesophilic lactic acid bacteria
ISO 15214:1998
10.
Lactobacillus acidophilus*ISO 20128:2006
11.
Mesophilic anaerobic microorganisms*
EVS-EN ISO 4833-1:2013, modified
12.
Mesophilic anaerobic spores*
ISO 15213:2003, modified
13.
Mesophilic aerobic spores*
EVS-EN ISO 15784:2009, modified
14.
Anaerobic sulphite reducing bacteria
ISO 15213:2003
* unaccredited method

II Detection of pathogens from food, feed and hygiene samples

Analysis
Method
1.
Salmonella spp (10g, 25g, 50g, 125g, 250g, 750g)
1) EVS-EN ISO 6579:2003/Cor.1: 2004
2) PCR method JU-MB-43, JU-MB-44
2.
Listeria monocytogenes (25 g)
1) EVS-EN ISO 11290- 1:2000/A1:2004
2) PCR method JU-MB-49, JU-MB-50
3.
Hygiene sample - Salmonella spp
1) EVS-EN ISO 6579:2003/Cor.1: 2004
2) PCR method JU-MB-43, JU-MB-44
4.
Hygiene sample - Listeria monocytogenes
1) EVS-EN ISO 11290- 1:2000/A1:2004
2) PCR method JU-MB-49, JU-MB-50
III Chemical analysis
1.Food pH determinationNMKL 179:2005


IV Microbiological analysis of air samples.

Name
Unit
1.
Aerobic plate count*
cfu/dish
2.
Yeasts and molds*
cfu/dish
* sedimentation method

V Bacterial Growth Media

Name
Unit
1.Mannitol egg yolk polymyxin agar (MYP)
Petri dish
2.Saboraud agar
Petri dish
3.Dichloran glycerol agar (DG18)
Petri dish
4.Plate count agar (PCA)
Petri dish
5.Tryptone soya agar (TSA)
Petri dish

VI Additional services

1.
Sensory analysis (JU-MB-42)*
2.
Identification of bacteria (sequencing)
3.
Comparative sample analysis
4.
Collection of food and hygiene samples
5.
Collection of drinking water samples
* unaccredited method

VII Consultation

  • Consultation on general food microbiology
  • Consultation on food hygiene for food preparation workers
  • Consultation on molecular biology for microbiologists in the food industry
    1. molecular diagnostics for detection of food-borne pathogens
    2. PCR method as a tool for food microbiologists




With any questions or concerns please contact our Head of Laboratory Mrs Ene Talpsep - ph: +372 737 7047 or ene.talpsep [at] icosagen.ee