Microbiological testing services
I Enumeration of microorganisms (cfu/g, cfu/ml) in food and feed samples
| Analysis | Method | |
| 1. | Total plate count | EVS-EN ISO 4833-1:2013 |
| 2. | Enterobacteriaceae | EVS-EN ISO 21528-2:2017 |
| 3. | β-glucuronidase positive Escherichia coli | EVS-ISO 16649-2:2011 |
| 4. | Coliforms** | ISO 4832:2006 |
| 5. | Coagulase-positive staphylococci (S. aureus etc) | EVS-EN ISO 6888-1:2001+A1+A2:2018 |
| 6. | Bacillus cereus | EVS-EN ISO 7932:2005 |
| 7. | Yeasts and molds | EVS-ISO 21527-1:2009 EVS-ISO 21527-2:2009 |
| 8. | Clostridium perfringens | EVS-EN ISO 7937:2004 |
| 9. | Listeria monocytogenes | EVS-EN ISO 11290-2:2017+/AC:2017 |
| 10. | Mesophilic lactic acid bacteria | ISO 15214:1998 |
| 11. | Mesophilic anaerobic microorganisms* | EVS-EN ISO 4833-1:2013, modified |
| 12. | Mesophilic anaerobic spores* | EVS-EN ISO 4833-1:2013, modified |
| 13. | Mesophilic aerobic spores* | EVS-EN ISO 15784:2009, modified |
| 14. | Anaerobic sulphite reducing bacteria | ISO 15213:2003 |
** scope of the accreditation: textile
II Enumeration of microorganisms (cfu/g, cfu/ml) in hygiene samples
| Analysis | Method | |
| 1. | Total plate count | EVS-EN ISO 4833-1:2013 |
| 2. | Enterobacteriaceae | EVS-EN ISO 21528-2:2017 |
| 3. | β-glucuronidase positive Escherichia coli | EVS-ISO 16649-2:2011 |
| 4. | Coliforms | ISO 4832:2006 |
III Detection of pathogens from food, feed, primary production stage and hygiene samples
| Analysis | Method | |
| 1. | Salmonella spp (10g, 25g, 50g, 125g, 250g, 750g) |
EVS-EN
ISO 6579-1:2017+A1:2020 2) PCR method JU-MB-43, JU-MB-44 |
| 2. | Listeria monocytogenes (25 g) | 1)
EVS-EN
ISO 11290-1:2017+/AC:2018 2) PCR method JU-MB-49, JU-MB-50 |
| 3. | Hygiene sample - Salmonella spp | 1)
EVS-EN
ISO 6579-1:2017+A1:2020
2) PCR method JU-MB-43, JU-MB-44 |
| 4. | Hygiene sample - Listeria monocytogenes | 1)
EVS-EN
ISO 11290-1:2017
2) PCR method JU-MB-49, JU-MB-50 |
| 5. | β-glucuronidase positive Escherichia coli** | ISO 16649-3:2015 |
| 6. | Coliforms | ISO 4831:2006 |
IV Chemical analysis
| Analysis | Method | |
| 1. | Food pH determination | ISO 2917; NMKL 179 |
| 2. | NaCl (through Na) | Ju-MB-59 |
| 3. | Humidity | JU-MB-60 |
V Microbiological analysis of water samples
| Analysis | Method | |
| 1. |
Escherichia coli
MPN method (Colilert-18)
| EVS-EN ISO
9308-2:2014
|
| 2. |
Coliforms, MPN method (Colilert-18)
|
EVS-EN ISO 9308-2:2014
|
| 3. | Intestinal Enterococci, MPN method (Enterlolert DW) |
JU-MB-62
|
| 4. |
Enumeration of culturable micro-organisms (22
°C)
|
EVS-EN ISO 6222:2001
|
| 5. |
Enumeration of culturable micro-organisms (37
°C)
|
EVS-EN ISO 6222:2001
|
VI Microbiological analysis of air samples.
| Name | Method | |
| 1. | Aerobic plate count* | JU-MB-52 |
| 2. | Yeasts and molds* | JU-MB-52 |
VII Bacterial Growth Media
| Name | Unit | |
| 1. | Mannitol egg yolk polymyxin agar (MYP) | Petri dish |
| 2. | Saboraud agar | Petri dish |
| 3. | Dichloran glycerol agar (DG18) | Petri dish |
| 4. | Plate count agar (PCA) | Petri dish |
| 5. | Tryptone soya agar (TSA) | Petri dish |
VIII Additional services
| Analysis | Method | |
| 1. | Food Sensory analysis* | JU-MB-42 |
| 2. | Identification of bacteria (16S rRNA sequencing)* | JU-MB-58 |
| 3. |
Collection of hygiene samples* | JU-MB-35 |
| 4. |
Collection of drinking water samples* |
JU-MB-41
|
| 5. |
Microscopy* | JU-MB-21 |
| 6. |
Microbiological growth media quality control* |
EVS-EN ISO 11133:2014+A1+A2:2020
|
| 7. |
Canned food sterility* | NMKL 59 (5):2004 |
| 8. | Interlaboratory comparison*
| |
| 9. | Transport |
IX Consultation
- Consultation on general food microbiology
- Consultation on food hygiene for food preparation workers
- Consultation on molecular biology for microbiologists in the food industry
- molecular diagnostics for detection of food-borne pathogens
- PCR method as a tool for food microbiologists
With any questions or concerns please contact our Head of Laboratory Mrs Ene Talpsep - ph: +372 737 7047 or ene.talpsep [at] icosagen.ee