List of services

Microbiological testing services

I Enumeration of microorganisms (cfu/g, cfu/ml) in food and feed samples

Analysis
Method
1.
Total plate count
EVS-EN ISO 4833-1:2013
2.
Enterobacteriaceae
EVS-EN ISO 21528-2:2017
3.
β-glucuronidase positive Escherichia coli
EVS-ISO 16649-2:2011
4.
Coliforms**
ISO 4832:2006
5.
Coagulase-positive staphylococci (S. aureus etc)
EVS-EN ISO 6888-1:2001+A1+A2:2018
6.
Bacillus cereus
EVS-EN ISO 7932:2005
7.
Yeasts and molds
EVS-ISO 21527-1:2009
EVS-ISO 21527-2:2009
8.
Clostridium perfringens
EVS-EN ISO 7937:2004
9.
Listeria monocytogenes
EVS-EN ISO 11290-2:2017+/AC:2017
10.
Mesophilic lactic acid bacteria
ISO 15214:1998
11.
Mesophilic anaerobic microorganisms*
EVS-EN ISO 4833-1:2013, modified
12.
Mesophilic anaerobic spores*
EVS-EN ISO 4833-1:2013, modified
13.
Mesophilic aerobic spores*
EVS-EN ISO 15784:2009, modified
14.
Anaerobic sulphite reducing bacteria
ISO 15213:2003
* unaccredited method
** scope of the accreditation: textile

II Enumeration of microorganisms (cfu/g, cfu/ml) in hygiene samples

Analysis
Method
1.
Total plate count
EVS-EN ISO 4833-1:2013
2.
Enterobacteriaceae
EVS-EN ISO 21528-2:2017
3.
β-glucuronidase positive Escherichia coli
EVS-ISO 16649-2:2011
4.
Coliforms
ISO 4832:2006
** scope of the accreditation: textile

III Detection of pathogens from food, feed, primary production stage and hygiene samples

Analysis
Method
1.
Salmonella spp (10g, 25g, 50g, 125g, 250g, 750g)
EVS-EN ISO 6579-1:2017+A1:2020
2) PCR method JU-MB-43, JU-MB-44
2.
Listeria monocytogenes (25 g)
1) EVS-EN ISO 11290-1:2017+/AC:2018
2) PCR method JU-MB-49, JU-MB-50
3.
Hygiene sample - Salmonella spp
1) EVS-EN ISO 6579-1:2017+A1:2020
2) PCR method JU-MB-43, JU-MB-44
4.
Hygiene sample - Listeria monocytogenes
1) EVS-EN ISO 11290-1:2017
2) PCR method JU-MB-49, JU-MB-50
5. β-glucuronidase positive Escherichia coli**  ISO 16649-3:2015
6.  Coliforms   ISO 4831:2006

IV Chemical analysis

  AnalysisMethod 
1.Food pH determinationISO 2917; NMKL 179
2.NaCl (through Na) Ju-MB-59 
3.Humidity JU-MB-60 

V Microbiological analysis of water samples

  Analysis Method
1. Escherichia coli MPN method (Colilert-18)
 EVS-EN ISO 9308-2:2014
2. Coliforms, MPN method (Colilert-18)
EVS-EN ISO 9308-2:2014
3. Intestinal Enterococci, MPN method (Enterlolert DW) JU-MB-62
4. Enumeration of culturable micro-organisms (22 °C)
EVS-EN ISO 6222:2001
5. Enumeration of culturable micro-organisms (37 °C)
EVS-EN ISO 6222:2001

VI Microbiological analysis of air samples.

Name
Method
1.
Aerobic plate count*
JU-MB-52
2.
Yeasts and molds*
JU-MB-52
* sedimentation method

VII Bacterial Growth Media

Name
Unit
1.Mannitol egg yolk polymyxin agar (MYP)
Petri dish
2.Saboraud agar
Petri dish
3.Dichloran glycerol agar (DG18)
Petri dish
4.Plate count agar (PCA)
Petri dish
5.Tryptone soya agar (TSA)
Petri dish

VIII Additional services

 AnalysisMethod
1.
Food Sensory analysis*
JU-MB-42
2.
Identification of bacteria (16S rRNA sequencing)*
JU-MB-58
3.
Collection of hygiene samples*
JU-MB-35
4.
Collection of drinking water samples*
JU-MB-41
5.
Microscopy*
JU-MB-21
6. Microbiological growth media quality control*
EVS-EN ISO 11133:2014+A1+A2:2020
7. Canned food sterility*
NMKL 59 (5):2004
8.Interlaboratory comparison*
  
9. Transport  
* unaccredited method

IX Consultation

  • Consultation on general food microbiology
  • Consultation on food hygiene for food preparation workers
  • Consultation on molecular biology for microbiologists in the food industry
  • molecular diagnostics for detection of food-borne pathogens
  • PCR method as a tool for food microbiologists




With any questions or concerns please contact our Head of Laboratory Mrs Ene Talpsep - ph: +372 737 7047 or ene.talpsep [at] icosagen.ee