SARS-CoV-2 Trimeric Spike UK VOC 202012/01 (alpha)
P-317-100
Datasheet as| Name | SARS-CoV-2 Trimeric Spike UK VOC 202012/01 |
| Description | Protein contains amino acids 14-1211 and following mutations: HV 69-70 del, Y144 del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain. |
| Uniprot ID |
P0DTC2
|
| Source | Human |
| MW | 410 kDa |
| Host |
CHO-based cell line (expressed by QMCF Technology)
|
| Purification |
Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
|
| Purity |
>95%
|
| Concentration | 1 mg/ml |
| Buffer |
PBS pH 7.4
|
| Endotoxine | NA |
| QC |
SDS-PAGE,
analytical SEC, Octet binding
|
| Shipping | Shipped on dry ice |
| Storage | Store at -70°C. Avoid multiple freeze-thaw cycles |
Buy now
€750 / 100µg
€1450 / 500µg
€1950 / 1000µg
Figure 1.
Simply Blue stained
SDS-PAGE analysis of
SARS-CoV-2 Trimeric Spike UK VOC 202012/01
. 4-12%
gradient gel is used for analysis. Lane 1. 1.3 µg
SARS-CoV-2 Trimeric Spike UK VOC 202012/01
(-DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg
SARS-CoV-2 Trimeric Spike UK VOC 202012/01
(+DTT)
Figure 2. Octet Red96e analysis of SARS-CoV-2 Trimeric Spike UK VOC 202012/01 binding to human ACE2 receptor.
Figure 2. Octet Red96e analysis of SARS-CoV-2 Trimeric Spike UK VOC 202012/01 binding to human ACE2 receptor.
