SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (alpha)
P-311-100
Datasheet as| Name | SARS-CoV-2 Spike RBD2 UK VOC 202012/01 |
| Source |
cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1)
Protein
contains amino acids 319-541, mutation N501Y, plus two extra amino acids (YS)
in N-terminus and His-6 tag at C-terminus and GSG linker between protein and
tag.
|
| MW | 26,33 kDa |
| Host |
CHO-based cell line (expressed by QMCF Technology)
|
| Purification |
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated
through 0.22 µm filter.
|
| Purity |
> 95%
|
| Concentration | 1 mg/ml |
| Buffer |
PBS pH 7.4
|
| Endotoxine | NA |
| QC |
SDS-PAGE NanoDrop A280 analytical SEC |
| Background |
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
|
| Shipping | Shipped on dry ice |
| Storage |
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
|
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Figure 1. SDS-PAGE analysis in reduced and non-reduced conditions. 1.3 µg of
SARS-CoV-2 Spike RBD2 UK VOC 202012/01
protein were loaded per lane.
Lane 1.
SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (reduced); Lane 2. Protein size marker (Smobio); Lane 3. SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (non-reduced)
Figure 2. Octet Red96e analysis of SARS-CoV-2 Spike RBD2 UK VOC 202012/01 binding to human ACE2 receptor.
SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (reduced); Lane 2. Protein size marker (Smobio); Lane 3. SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (non-reduced)
Figure 2. Octet Red96e analysis of SARS-CoV-2 Spike RBD2 UK VOC 202012/01 binding to human ACE2 receptor.
