SARS-CoV-2 Spike RBD2 Brazil P1 (gamma)
P-315-100
Datasheet as| Name | SARS-CoV-2 Spike RBD2 Brazil P1 |
| Source |
cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1) Protein contains amino acids 319-541, mutations K417T, E484K, N501Y, plus two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag. |
| MW | 28.4 kDa |
| Host |
CHO-based cell line (expressed by QMCF Technology)
|
| Purification |
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated
through 0.22 µm filter.
|
| Purity |
>95%
|
| Concentration | 1 mg/ml |
| Buffer |
PBS pH 7.4
|
| Endotoxine | NA |
| QC |
Coomassie
stained SDS-PAGE, analytical SEC
|
| Shipping | Shipped on dry ice |
| Storage |
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
|
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€500 / 100µg
€1000 / 500µg
€1500 / 1000µg
Figure 1. Coomassie-stained SDS-PAGE
analysis of SARS-CoV-2 Spike RBD2 Brazil P1. 4-15% gradient gel is used for
analysis.
Lane 1. 1.3 µg
SARS-CoV-2 Spike RBD2 Brazil
P1
(+DTT)
Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg
SARS-CoV-2 Spike RBD2 Brazil
P1
(+DTT)
Figure 2. Octet Red96e analysis of SARS-CoV-2 Spike RBD2 Brazil P1 protein binding to human ACE2 receptor.
Figure 2. Octet Red96e analysis of SARS-CoV-2 Spike RBD2 Brazil P1 protein binding to human ACE2 receptor.
