SARS-CoV-2 Spike S1
P-305-100
Datasheet as| Name | SARS-CoV-2 Spike S1 |
| Description | cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1) Protein contains amino acids 14-681, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus. |
| MW |
76,42 kDa
|
| Host |
CHO-based cell line (expressed by QMCF Technology)
|
| Purification |
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated
through 0.22 µm filter.
|
| Purity | >90% |
| Concentration | 1 mg/ml |
| Buffer |
PBS pH 7.4
|
| Endotoxine | NA |
| Bioproperties | Measured by its binding ability to ACE2 protein by OCTET RED96 system. |
| QC |
SDS-PAGE NanoDrop A280 |
| Shipping |
Shipped on dry-ice
|
| Storage |
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
|
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Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-COV-2 S1. 4-15% gradient gel is used for analysis.
Figure 2. Binding analysis of the SARS-CoV-2 Spike S1 protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 25 to 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.
Figure 2. Binding analysis of the SARS-CoV-2 Spike S1 protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 25 to 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.
