SARS-CoV-2 S1 RBD
P-303-100
Datasheet as| Name | SARS-CoV-2 S1 RBD |
| Source |
cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1)
Protein contains amino acids 331-524, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus. |
| MW |
23 kDa
|
| Host |
CHO-based cell line (expressed by QMCF Technology)
|
| Purification |
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated
through 0.22 µm filter.
|
| Purity | >95% |
| Concentration | 1 mg/ml |
| Buffer |
PBS pH 7.4
|
| Endotoxine | NA |
| Bioproperties |
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
|
| QC | SDS-PAGE NanoDrop A280 |
| Shipping |
Shipped on dry-ice
|
| Storage |
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
|
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Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-COV-2 S1 RBD. 4-15% gradient gel is used for analysis
Figure 2. Binding analysis of the SARS-CoV-2 S1 RBD protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 25 to 200 nanomols of SARS-CoV-2 S1 RBD protein was bound to the ACE2 receptor.
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Figure 2. Binding analysis of the SARS-CoV-2 S1 RBD protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 25 to 200 nanomols of SARS-CoV-2 S1 RBD protein was bound to the ACE2 receptor.
