Mouse monoclonal antibody to human GDNF
315-100
Datasheet as| Name | Mouse
monoclonal antibody to human GDNF |
| Immunogen | Human GDNF |
| Immunogen Description | Recombinant human GDNF protein produced using E. coli expression system |
| Uniprot ID | P39905 |
| Alternative Names | Astrocyte-derived trophic factor (ATF) |
| Clonality | Mouse monoclonal |
| Clone | 3C1 |
| Class | murine IgG1 |
| Reactivity | Human GDNF |
| Application | ELISA, WB, IF, IHC |
| Protocol | ELISA 50 to 100 ng/ml; Western immunoblotting 2 to 5 µg/ml ; IF 2 to 10 µg/ml; IHC 5 µg/ml. Monoclonal antibody working amount has to be established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Monoclonal and polyclonal antibodies to human GDNF. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into
aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | GDNF is a neurotrophic factor that enhances survival
and morphological differentiation of dopaminergic neurons and increases their
high-affinity dopamine uptake. Ligand for the GFR-alpha-3-RET receptor complex
but can also activate the GFR-alpha-1-RET receptor complex |
| References | - |
| Limitations | This
product is for research use only |
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€299 / 100µg
Figure
1. Western
Blot testing of GDNF monoclonal antibody 3C1. Analysis was performed
with antiGDNF monoclonal antibody 3C1 HRP conjugate. 15 µl of CHOEBNALT85 GDNF
producing cell culture supernatant was loaded per lane. Analysis was performed
in reducing (A) and non-reducing (B) conditions
A
B
Figure 2. Immunofluorescence
detection of human GDNF expressed in U2OS cells
by anti-GDNF monoclonal antibody 3C1. Anti-GDNF antibody 3C1
concentration in IF experiment was 10 µg/ml. Goat ant-mouse AlexaFluor488 was
used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI
platform (Thermo Scientific) was used for image acquisition (10x objective). Composite
picture was generated using pseudocolors green for GDNF specific signal and
blue for nuclei. A. GDNF-expressing U2OS cells; B. Negative
control (non-transfected U2OS cells)
A
B
Figure
3. Immunohistochemistry testing of anti-GDNF monoclonal antibody 3C1. Analysis
was performed using formalin-fixed paraffin-embedded human cerebral cortex
tissue sections from Alzheimer’s disease patients. Tissue sections were boiled
with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with
primary antibody at 5 µg/ml was performed overnight at 4°C. DAKO EnVisionTM
Detection System, Peroxidase/DAB was used for visualization. Sections were
counterstained with toluidine blue and mounted with Eukitt mounting
medium. A. GDNF staining by monoclonal antibody 3C1; B.Negative
staining without primary antibody