Human monoclonal scFv-Fc antibody to Zaire Ebolavirus GP protein


Datasheet as
Name Human monoclonal scFv-Fc antibody to Zaire Ebolavirus GP protein
Immunogen Ebolavirus GP protein
Immunogen Description Envelope glycoprotein processed to GP1 and GP2. Transmembrane and cytoplasmatic domains are removed. Immunogen contains C-terminal linker and hexahistidine tag sequence (GSGHHHHHH). Expressed by 293-based cell line (expressed by QMCF Technology)
Uniprot ID A0A068J419
Alternative Names Virion spike glycoprotein, GP protein
Clonality Monoclonal: rabbit scFv, human Fc
Clone 7H7
Class hIgG1 scFv-Fc
Reactivity Zaire Ebolavirus GP protein
Dissociation constant (KD) 1.2 x 10-10 M
Application ELISA, WB, IF
Protocol ELISA 25 - 100 ng/ml
WB 0.5 - 1 µg/ml
IF 0.156 - 5 µg/ml
Purification Protein A affinity purification
Buffer PBS pH 6.0
Unit Size 100 µg
Related Products Rabbit polyclonal antibody to Zaire Ebolavirus GP protein
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background GP1 is responsible for binding to the receptors on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. Binding to CD209 and CLEC4M, which are respectively found on dendritic cells (DCs), and on endothelial cells of liver sinusoids and lymph node sinuses, facilitate infection of macrophages and endothelial cells. These interactions not only facilitate virus cell entry, but also allow capture of viral particles by DCs and subsequent transmission to susceptible cells without DCs infection (trans infection). GP2 acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. Mediates activation of primary macrophages. At terminal stages of the viral infection, when its expression is high, GP1,2 down-modulates the expression of various host cell surface molecules that are essential for immune surveillance and cell adhesion. Down-modulates integrins ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGAV and ITGB1. GP1,2 alters the cellular recycling of the dimer alpha-V/beta-3 via a dynamin-dependent pathway. Decrease in the host cell surface expression of various adhesion molecules may lead to cell detachment, contributing to the disruption of blood vessel integrity and hemorrhages developed during Ebola virus infection (cytotoxicity). (Ebola GP1 and 2 functions, UniProt).
Limitations This product is for research use only


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Figure 1. Western-Blot analysis of 7H7 scFv-hFc antibody binding to Zaire Ebolavirus GP2 subunit (Lanes 1-3). As a control, rabbit polyclonal antibody to Zaire Ebolavirus GP (A2-100-100) binds both GP1 and GP2 subunits (Lane 8). No binding of 7H7 (Lanes 4-6) and A2-200-100 (Lane 9) to Sudan Ebolavirus GP2 subunit is detected. Proteins loaded: 200 ng (Lanes 1, 4), 100 ng (Lanes 2, 5), 20 ng (Lanes 3, 6). Lanes 7, 10, Prestained Protein Ladder, Naxo 8003. Primary antibody dilution 1 µg/ml.

Figure 2. 7H7 scFv-hFc binding to Zaire GP-mut#56 transfected (left) and mock-transfected (right) U2OS cells. 7H7 concentration 0.156 µg/ml. Fluorescence was measured at corresponding wavelength of DAPI and secondary antibody (Goat pAb to human IgG DyLight 549)