Rabbit anti-chicken polyclonal antibody DyLight 488 conjugate
904-100
Datasheet as| Name | Rabbit anti-chicken polyclonal antibody DyLight 488 conjugate |
| Immunogen | chicken IgY |
| Immunogen Description | Purified IgY fraction |
| Clonality | Rabbit polyclonal, DyLight 488 conjugate |
| Class | IgG |
| Reactivity | Reacts with chicken IgY antibodies |
| Application | IF |
| Protocol | IF: 5 to 10 µg/ml Polyclonal antibody working titer has to be
established practically for each particular antigen and assay format |
| Purification | IgG fraction purified
by IgY affinity chromatography |
| Buffer | PBS pH 7.4, with 50% glycerol |
| Unit Size | 100 µg |
| Related Products | Rabbit-anti chicken polyclonal antibodies, different
conjugates. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in frozen format. |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into
aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Limitations | This
product is for research use only |
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€299 / 100µg
Figure
1. Immunofluorescent analysis for testing of
Rabbit anti-chicken polyclonal antibody DyLight 488 conjugate as secondary
antibody. Chicken polyclonal antibody to human GFRα-3 (305-100
Icosagen), dilution 1:3000 was used as primary antibody. For nuclear staining
DAPI was used. A. U2OS cells
expressing GFRα-3. B. Non-transfected
U2OS cells. ArrayScan VTI platform (Thermo Scientific) was used for image
acquisition (10x objective)