Chicken polyclonal antibody to human GFRa-4
308-100
Datasheet as| Name | Chickenpolyclonal antibody to human GFRa-4 |
| Immunogen | human GFRa-4 |
| Immunogen Description | Recombinant His-tagged human GFRa-4 protein produced
using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed
and protein was secreted to the cell culture supernatant. Protein was purified
by Ni-affinity chromatography following gel-filtration from cell culture
supernatant |
| Uniprot ID | Q9GZZ7 |
| Alternative Names | GDNF receptor alpha-4, Persephin receptor |
| Clonality | Chicken polyclonal |
| Class | IgY |
| Reactivity | Human GFRa-4, doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-3 |
| Application | ELISA, WB, IF |
| Protocol | WB 0.1 to 0.2 µg/ml; ELISA: 3 to 6.7 ng/ml; IF 0.3 to 1 µg/ml Polyclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | GFRa-4 affinity purified |
| Buffer | PBS pH 7.4 |
| Unit Size | 100 µg |
| Related Products | Mono- and polyclonal antibodies to human neural growth factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
|
| Background | GFRα-4 belongs to the GDNF receptor family. It is a
glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin,
and mediates activation of the RET tyrosine kinase receptor |
| Limitations | This
product is for research use only |
Figure 1. IF detection of human GFRα-4 expressed in U2OS
cells. GFRα-4 was visualized using Chicken polyclonal antibody to human GFRα-4
(dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam,
ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was
used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition
(10x objective). Composite picture was generated using pseudocolors green for
GFRα-4 specific signal and blue for nuclei
Figure
2. Western-Blot
detection of human GFRα-4, using Chicken polyclonal antibody to human GFRα-4. Protein
was expressed by CHO cell culture. 100 ng of purified protein was loaded per
lane. Line 1. hGFRα-4. Primary
antibody dilution 1:5000 was used. Line
2. Prestained protein ladder (Naxo 8003). Rabbit anti-chicken antibody, HRP
conjugate (903-100, Icosagen) was used as secondary antibody