Rabbit anti-chicken polyclonal antibody HRP conjugate
903-100
Datasheet as| Name | Rabbit anti-chicken polyclonal antibody HRP conjugate |
| Immunogen | chicken IgY |
| Immunogen Description | Purified IgY fraction |
| Clonality | Rabbit polyclonal, HRP conjugate |
| Class | IgG |
| Reactivity | Reacts with chicken IgY antibodies |
| Application | ELISA, WB |
| Protocol | ELISA: 0.03 to 0.05 µg/ml, WB: 0.1 to 0.2 µg/ml Polyclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | IgG fraction purified
by IgY affinity chromatography |
| Buffer | PBS pH 7.4, with 50% glycerol |
| Unit Size | 100 µg |
| Related Products | Rabbit-anti chicken polyclonal antibodies, different
conjugates. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in frozen format |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into
aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Limitations | This
product is for research use only |
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€299 / 100µg
Figure
1.
Western blot analysis for testing of
Rabbit anti-chicken polyclonal antibody HRP conjugate as secondary antibody.
Chicken anti-MANF polyclonal antibody (Icosagen) was used as primary antibody.
For secondary antibody testing purified human MANF protein was used. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified MANF protein. 100 ng of protein is loaded per lane