Chicken polyclonal antibody to human GFRa-2
304-100
Datasheet as| Name | Chicken
polyclonal antibody to human GFRa-2 |
| Immunogen | human GFRa-2 |
| Immunogen Description | Recombinant His-tagged human GFRα-2 protein produced
using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
For production of hGFRα-2, glycosylphosphatidyl-inositol GPI-anchor was removed
and protein was secreted to the cell culture supernatant. Protein was purified
by Ni-affinity chromatography following gel-filtration from cell culture
supernatant |
| Uniprot ID | O00451 |
| Alternative Names | GDNF receptor beta, Neurturin receptor alpha, RET
ligand 2 TGF-beta-related neurotrophic factor receptor 2 |
| Clonality | Chicken polyclonal |
| Clone | - |
| Class | IgY |
| Reactivity | Human GFRa-2, doesn’t cross-react with hGFRa-1, hGFRa-3, hGFRa-4 |
| Application | ELISA, WB, IF |
| Protocol | WB 0.2 to 1 µg/ml; ELISA: 6.25 to 8 ng/ml; IF 1 to 2 µg/ml Polyclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | GFRa-2 affinity purified |
| Buffer | Concentrated ammonium sulphate in PBS pH 7.4 |
| Unit Size | 100 µg |
| Related Products | Mono- and polyclonal antibodies to human growth
factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at +4°C upon receipt. As product is (NH4)2SO4
precipitate, mix well by pipetting or vortexing prior use |
| Background | Glial cell line-derived neurotrophic factor (GDNF) and
neurturin (NTN) are two structurally related, potent neurotrophic factors that
play key roles in the control of neuron survival and differentiation. GFRa-2 is
a member of the GDNF receptor family. It is a glycosylphosphatidyl-inositol(GPI)-linked
cell surface receptor for both GDNF and NTN, and mediates activation of the RET
tyrosine kinase receptor. This encoded protein acts preferentially as a
receptor for NRTN compared to its other family member, GDNF family receptor
alpha 1. http://www.ncbi.nlm.nih.gov/gene/2675 |
| References | - |
| Limitations | This
product is for research use only |
Figure 1. IF detection of human GFRα-2 expressed in U2OS
cells. GFRα-2 was visualized using Chicken polyclonal antibody to human GFRα-2
(dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam,
ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was
used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition
(10x objective). Composite picture was generated using pseudocolors green for
GFRα-2 specific signal and blue for nuclei.
Figure 2. Western-Blot detection of human GFRα-2, using Chicken polyclonal antibody to human GFRα-2. GFRα-2 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-2. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody (Icosagen) was used as secondary antibody