Mouse monoclonal antibody to bovine DNase I


Datasheet as
Name Mouse monoclonal antibody to bovine DNase I
Immunogen Bovine DNase I
Immunogen Description Recombinant full length bovine DNaseI produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant
Uniprot ID P00639
Alternative Names DNL1
Clonality Mouse monoclonal
Clone 4B10
Class IgG1
Reactivity Bovine, cross reactivity to Human
Application ELISA, WB
Protocol ELISA 0.01 to 0.02 µg/ml; WB 0.1 to 0.2 μg/ml (bovine DNase I)
Antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products Bovine DNase I polyclonal antibody, cat no 400-100
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments
Limitations This product is for research use only 


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€323 / 100µg
€1292 / 500µg
€2098 / 1000µg

Figure 1. Western Blot testing of anti-bovine DNaseI monoclonal antibody (4B10).
Lane 1. Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium. 10 µl of cell culture supernatant was loaded into the gel. Lane 2. PageRuler Prestained Protein Ladder (#SM0671 Fermentas);