Partially humanized, chimeric recombinant monoclonal antibody to HIV-1 p24
C-851-100
Datasheet as| Name | Partially humanized, chimeric recombinant monoclonal antibody to HIV-1 p24 |
| Immunogen | HIV-1 p24 |
| Immunogen Description | Recombinant p24 protein of HIV-1 HAN consensus (purified from E. coli) |
| Clonality | Partially humanized monoclonal |
| Clone | 5 |
| Class | IgG1 |
| Reactivity | Reacts with HIV-1 p24 subtype B, C and A. Mapped to 48-62(TPQDLNTMLNTVGGH) |
| Application | ELISA, WB |
| Protocol |
WB 1 µg/ml; IF 1 to 10 µg/ml Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. For detection please use anti-human IgG antibody |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, 50% glycerole |
| Unit Size | 100 µg |
| Related Products | Mouse anti-HIV 1 p24 monoclonal antibody clone 5 A2-851-100. HIV-1 p24 monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | Antibody is derived from mouse anti-HIV-1 (HAN subtype) p17/24 monoclonal antoibody clone 5 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 05-001 epitope-tag sequence. For more information please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
Figure 1: Western-Blot analysis of HIV-1 HAN subtype p17/24 protein using chimeric HIV-1 p24 antibody, clone 5. Line 1. Protein size marker Line 2. HIV-1 p17/24 20 ng per lane
Figure 2: IF analysis using chimeric HIV-1 p24 antibody, clone 5. ETAR (Endothelin A receptor) is fused with 05-001 epitope tag, expressed in CHOEBNALT85 cell line and detected in IF assay