Rabbit polyclonal antibody to human MANF
310-100
Datasheet as| Name | Rabbit polyclonal antibody to human MANF (Mesencephalic astrocyte-derived neurotrophic factor) |
| Immunogen | Human MANF |
| Immunogen Description | Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant |
| Uniprot ID | P55145 |
| Alternative Names | ARMET, ARP |
| Clonality | Rabbit polyclonal |
| Clone | - |
| Class | IgG |
| Reactivity | Human MANF |
| Application | ELISA, WB, IF, IHC |
| Protocol |
ELISA 0.1 to 0.2 µg/ml; WB 0.15 to 0.5 µg/ml, IHC 10 µg/ml, IF 1 to 20 µg/ml No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. Polyclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | MANF-affinity purified |
| Buffer | Concentrated ammonium sulphate in PBS pH 7.4. |
| Unit Size | 100 µg |
| Related Products | Monoclonal and polyclonal antibodies to human MANF. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use |
| Background | MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta |
| References | - |
| Limitations | This product is for research use only |
Figure 1. Western Blot testing of anti-MANF polyclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (1 µg per line) expressed using CHOEBNALT85 cell line
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Figure 2: Immunohistochemical analysis of rabbit polyclonal antibody to human MANF. Analysis was performed using 4-5 µm FFPE human cerebral cortex tissue sections from Alzheimer’s disease patients. Antibody concentration of 10 µg/ml was used. A. MANF staining by polyclonal antibody. B. negative staining.
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Figure 3. Immunofluorescence detection of human MANF expressed in human U2OS cells by rabbit polyclonal antibody to hMANF. Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. A. MANF-expressing U2OS cells. B. negative control (non-transfected cells)