Mouse monoclonal antibody to HIV-1 Tat
A2-827-100
Datasheet as| Name | Mouse monoclonal antibody to HIV-1 Tat |
| Immunogen | HIV-1 Tat |
| Immunogen Description | Recombinant Tat protein of HIV-1 HAN (purified from E. coli) |
| Clonality | Mouse monoclonal |
| Clone | N8 |
| Class | IgG2b |
| Reactivity | Reacts in ELISA and WB with HIV-1 Tat of HXB2, BH10, IIIB and HAN strains. Mapped to amino acids 59-67 (PQDSQTHQV – B subtype a.a. consensus) |
| Application | ELISA, WB, ChIP |
| Protocol | ELISA 0.05 to 0.1 µg/ml; WB 2 µg/ml. Monoclonal antibody working
titer has to be established practically for each particular antigen and assay
format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20…-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
Figure
1. Western Blot testing of HIV-1 recombinant Tat
protein. using HIV-1 Tat antibody (clone N8). Lane 1. Recombinant HIV-1
Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml. Lane 2. Protein size
marker
Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone N8). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA