Mouse monoclonal antibody to HIV-1 Tat
A2-823-100
Datasheet as| Name | Mouse monoclonal antibody to HIV-1 Tat |
| Immunogen | HIV-1 Tat |
| Immunogen Description | Recombinant Tat protein of HIV-1 BH10 (purified from E. coli) |
| Clonality | Mouse monoclonal |
| Clone | 15 |
| Class | IgG1 |
| Reactivity | Reacts HIV-1 Tat of BH10, IIIB strains. HIV-1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE). Mapped to amino acids 2-9 (EPVDPRLE - B subtype a.a. consensus) |
| Application | ELISA, WB, ChIP |
| Protocol | ELISA 0.16 to 0.8 µg/ml; WB 2 µg/ml Monoclonal antibody
working titer has to be established practically for each particular antigen and
assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20…-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| References |
Campioni, D., Corallini, A., Zauli, G., Possati, L., Altavilla, G. and Barbanti-Brodano, G (1995). HIV type I extracellular Tat protein stimulates growth and protects cells of BK virus/tat transgenic mice from apoptosis. AIDS Res Hum Retroviruses 11, 1039-1048. PMID: 8554901 Huang L, Bosch I, Hofmann W, Sodroski J, Pardee AB. (1998). Tat protein induces human immunodeficiency virus type 1 (HIV-1) coreceptors and promotes infection with both macrophage-tropic and T-lymphotropic HIV-1 strains. J Virol. 72(11):8952-60. PMID: 9765440 Rohr O, C Schwartz, C Hery, D Aunis, M Tardieu and E Schaeffer (2000). The nuclear receptor COUP-TF interacts with HIV-1 Tat and stimulates viral replication in human microglial cells. Journal of Biological Chemistry. 28;275(4): 2654-60. PMID: 10644726 Arp J, Rieder MJ, Urquhart B, Freeman D, Tucker MJ, Krizova A, Lehmann D, Dekaban GA (2005). Hypersensitivity of HIV-1 infected cells to reactive sulfonamide metabolites correlated to expression of the HIV-1 viral protein tat. J. Pharmcol Exp Ther 314:1218-25. PMID: 15933158 Karasev AV, Foulke S, Wellens C, Rich A, Shon KJ, Zwierzynski I, Hone D, Koprowski H, Reitz M. (2005). Plant based HIV-1 vaccine candidate: Tat protein produced in spinach. Vaccine. 7;23(15):1875-80. PMID: 15734059 Abbott KL, Archambault J, Xiao H, Nguyen BD, Roeder RG, Greenblatt J, Omichinski JG, Legault P. (2005) Interactions of the HIV-1 Tat and RAP74 Proteins with the RNA Polymerase II CTD Phosphatase FCP1. Biochemistry 44, 2716-2731. PMID: 15723517 Rolland M, Jensen MA, Nickle DC,Yan J, Learn GH,1 Heath L, Weiner D, Mullins JI.(2007) Reconstruction and Function of Ancestral Center-of-Tree Human Immunodeficiency Virus Type 1 Proteins. Journal of Virology, Vol. 81, No. 16 |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
€1195 / 500 µg
€1942 / 1000 µg
Figure 1. Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone 15). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml. Lane 2. Protein size marker
