Mouse monoclonal antibody to HIV-1 Nef


Datasheet as
Name Mouse monoclonal antibody to HIV-1 Nef
Immunogen HIV-1 Nef
Immunogen Description

Recombinant Nef protein of HIV-1 HXB2 (purified from E. coli)

Uniprot ID -
Alternative Names 3’ORF
Clonality Mouse monoclonal
Clone 3D12
Class IgG1

Reacts with HIV-1 subtypes B and C, BRU, IIIB, and HAN strains. Mapped to amino acids 35-50 (RDLEKHGAITSSNTAA - HXB2)

Application ELISA, WB, ICC
Protocol ELISA 0.1 to 0.2 µg/ml; WB 2 µg/ml. 
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Protein G purification

Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products

HIV-1 Nef monoclonal antibodies. For more information visit

Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20… -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background -

Ovod V., Lagerstedt, A., Ranki, A., Gombert, F.O., Spohn, R., Tahtinen, M., Jung, G., Krohn K.J. 1992. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS, 6:25-34.

Saito, Y., L. R. Sharer, L. G. Epstein, J. Michaels, M. Mintz, M. Louder, K. Golding, T. A. Cvetkovich, and B. M. Blumberg. 1994. Overexpression of Nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology 44:474-481

Ranki A. , M. Nyberg, V. Ovod, M. Haltia, I. Elovaara, R. Raininko, H. Haapasalo, and K. Krohn. 1995. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS, 9:1001-1008.

Collings, A., Pitkanen, J., Strengell, M., Tahtinen, M., Lagerstedt, A., Hakkarainen, K., Ovod, V., Sutter, G., Ustav, M., Ustav, E., Mannik, A., Ranki, A., Peterson, P., Krohn, K. 1999. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 18(5- 6):460-7.

Kjerrström, A., J. Hinkula, G.Engström, V.Ovod, K.Krohn, R.Benthin and B.Wahren. 2001. Interactions of Single and Combined Human Immunodeficiency Virus Type 1 (HIV-1) DNA Vaccines. Virology 284(1): 46-61.

Maksiutov A.Z. , A.G. Bachinskii, and S.I. Bazhan. 2002 [Searching for Local Similarities Between HIV-1 and Human Proteins. Application to Vaccines]. Mol Biol (Mosk), 36(3):447-459.

Lehmann M.H., Walter S, Ylisastigui L, Striebel F, Ovod V, Geyer M, Gluckman JC, Erfle V. 2006 Extracellular HIV-1 Nef increases migration of monocytes. Exp Cell Res. 312 (18):3659-68.


This product is for research use only


Please refer any technical questions to

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€323 / 100µg
€1292 / 500µg
€2098 / 1000µg

Figure 1:
 Western Blot testing of HIV-1 recombinant Nef protein. using HIV-1 Nef antibody, clone 3D12 (01-002). Line 1. Recombinant HIV-1 Nef (B subtype) Line 2. Protein size marker.