Mouse monoclonal antibody to HCV subtype 1b NS5Bprotein


Datasheet as
Name Mouse monoclonal antibody to HCV subtype 1b NS5B
Immunogen HCV 1b NS5B
Immunogen Description Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)
Uniprot ID Q9WMX2
Clonality Mouse monoclonal
Clone 6B12
Class IgG1
Reactivity Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 92-105 (TPPHSARSKFGYGA)
Application ELISA, WB, IP

WB in reduced conditions 1 µg/ml; 

Optimal conditions for IP should be determined for each particular application.

Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HCV subtype 1b monoclonal antibodies. For more information visit
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Background Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.
References Nikonov A, Juronen E, Ustav M (2008).  Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.
Limitations This product is for research use only 


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€323 / 100µg
€1292 / 500µg
€2098 / 1000µg

Figure 1. Immunoprecipitation analysis of anti-HCV 1b NS5B monoclonal antibody (6B12). IP was carried out with NS5B specific mAb 6B12 using the lysates of Huh7 cells harbouring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (a-NS5B) and a-Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with NS5B specific mAb 10D6.

Figure 2. WB analysis of Huh-7 cell line expressing NS5B protein. Lane 1. Huh-7 transfected with HCV replicon, Lane 2. Huh-7 non-transfected cells.