Mouse monoclonal antibody to HCV subtype 1b NS5Bprotein
A2-421-100
Datasheet as| Name | Mouse monoclonal antibody to HCV subtype 1b NS5B protein |
| Immunogen | HCV 1b NS5B |
| Immunogen Description |
Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp) |
| Uniprot ID | Q9WMX2 |
| Clonality | Mouse monoclonal |
| Clone | 6G5 |
| Class | IgG1 |
| Reactivity | Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 77-86 (TVKAKLLSVE) |
| Application | ELISA, WB, IP |
| Protocol |
WB in reduced conditions 1 µg/ml; Optimal conditions for IP should be determined for each particular application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Background | Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. |
| References | Nikonov A, Juronen E, Ustav M (2008). Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102. |
| Limitations | This product is for research use only |
Figure 1. IP was carried out with NS5B specific mAb 6G5 using the lysates of Huh7 cells harboring selectable subgenomic HCV RNA replicon (upper panel) or plain Huh7 cells (lower panel). NS5B polyclonal antibodies (a-NS5B) and a-Bcl2 mAb, directed against cellular protein, were used as positive and negative controls respectively. Asterisk indicates immunoglobulin heavy chain; protein blots were probed with NS5B specific mAb 10D6.
Figure 2. WB analysis of Huh-7 cell line expressing NS5B protein. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.