Mouse monoclonal antibody to HCV subtype 1b NS5Bprotein
A2-426-100
Datasheet asName | Mouse monoclonal antibody to HCV subtype 1b NS5B protein |
Immunogen | HCV 1b NS5B |
Immunogen Description |
Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp) |
Uniprot ID | Q9WMX2 |
Alternative Names | - |
Clonality | Mouse monoclonal |
Clone | 10D7 |
Class | IgG1 |
Reactivity | Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 92-105 (TPPHSARSKFGYGA) |
Application | ELISA, WB |
Protocol |
WB in reduced conditions 1 µg/ml; Optimal conditions for IP should be determined for each particular application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
Purification | Protein G purification |
Buffer | PBS pH 7.4, with 0.1% sodium azide |
Unit Size | 100 µg |
Related Products | HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
Storage |
Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
Background |
Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. |
References | Nikonov A, Juronen E and Ustav M (2008). Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102. |
Limitations | This product is for research use only |
€323 / 100µg
€1292 / 500µg
€2098 / 1000µg
Figure 1. WB analysis of Huh-7 cell line expressing NS5B protein. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.