Mouse monoclonal antibody to HCV subtype 1b NS5Bprotein
|Name||Mouse monoclonal antibody to HCV subtype 1b NS5B protein
|Immunogen||HCV 1b NS5B|
Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)
|Reactivity||Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 92-105 (TPPHSARSKFGYGA)|
WB in reduced conditions 1 µg/ml;
Optimal conditions for IP should be determined for each particular application.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
|Purification||Protein G purification
|Buffer||PBS pH 7.4, with 0.1% sodium azide|
|Unit Size||100 µg|
|Related Products||HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies|
|Shipping||This product is shipped in non-frozen liquid form in ambient conditions|
Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.
|References||Nikonov A, Juronen E and Ustav M (2008). Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.
This product is for research use only
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Figure 1. WB analysis of Huh-7 cell line expressing NS5B protein. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.