Mouse monoclonal antibody to HCV subtype 1b NS5Bprotein
A2-426-100
Datasheet as| Name | Mouse monoclonal antibody to HCV subtype 1b NS5B protein |
| Immunogen | HCV 1b NS5B |
| Immunogen Description |
Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp) |
| Uniprot ID | Q9WMX2 |
| Alternative Names | - |
| Clonality | Mouse monoclonal |
| Clone | 10D7 |
| Class | IgG1 |
| Reactivity | Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 92-105 (TPPHSARSKFGYGA) |
| Application | ELISA, WB |
| Protocol |
WB in reduced conditions 1 µg/ml; Optimal conditions for IP should be determined for each particular application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | HCV subtype 1b monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage |
Store at - 20 …-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Background |
Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. |
| References | Nikonov A, Juronen E and Ustav M (2008). Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102. |
| Limitations | This product is for research use only |
Figure 1. WB analysis of Huh-7 cell line expressing NS5B protein. Line 1. Huh-7 transfected with HCV replicon, Line 2. Huh-7 non-transfected cells.