Mouse monoclonal antibody to human CDNF
301-100
Datasheet as| Name | Mouse monoclonal antibody to human CDNF |
| Immunogen | Human CDNF |
| Immunogen Description | Recombinant human CDNF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Purified from cell culture supernatant |
| Uniprot ID | Q49AH0 |
| Alternative Names | ARMETL1 |
| Clonality | Mouse monoclonal |
| Clone | 7D6 |
| Class | IgG1 |
| Reactivity | Human, mouse;
others not tested Binds to the N-terminal part of the human CDNF (aa 25-125) |
| Application | ELISA, WB, IF |
| Protocol | ELISA (rhCDNF) 0.1 to 0.2 µg/ml; ELISA (rmCDNF) 0.13 to 0.4 µg/ml; WB 0.25 to 1 µg/ml; IF 0.33 to 10 µg/ml; Monoclonal antibody working dilution has to be established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Mono- and polyclonal antibodies to human CDNF. For more details www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20 °C to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result |
| Background | CDNF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson’s disease. When administered after 6-OHDA or MPTP –lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta |
| References | - |
| Limitations |
This product is for research use only |
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€299 / 100µg
Figure 1. Western Blot testing of anti-CDNF monoclonal antibody (7D6). Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant CDNF expressed into the supernatant of CHO cell culture medium.
A
B
Figure
2. Immunofluorescence detection of human CDNF
expressed in U2OS cells. CDNF was visualized using anti-CDNF antibody clone
7D6 at 1 µg/ml. Goat ant-mouse
AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was
used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition
(10x objective). Composite picture was generated using pseudocolors green for
CDNF specific signal and blue for nuclei. A.
CDNF-expressing U2OS cells; B. Negative
control