Mouse monoclonal antibody to Bovine Papillomavirus type 1E2-protein
A1-101-100
Datasheet as| Name | Mouse monoclonal antibody to Bovine Papillomavirus type 1 E2-protein |
| Immunogen | BPV type 1 E2 |
| Immunogen Description | Recombinant full length BPV type 1 E2 protein purified from E. coli |
| Uniprot ID | P03122 |
| Clonality | Mouse monoclonal |
| Clone | 3F12 |
| Class | IgG |
| Reactivity | Bovine, BPV type 1 E2 protein, mapped to amino acids 197-208 (GVSSTSSDFRDR) |
| Application | ELISA, WB, IF |
| Protocol |
ELISA 0.05 to 0.1 µg/ml; WB 0.33 to 1 µg/ml; IF 1 µg/ml Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Purification | Protein A purification |
| Buffer | PBS with 50% glycerol |
| Unit Size | 100 µg |
| Related Products | 9 different monoclonal antibodies to BPV type 1 E2 protein. For more information and ordering see full list: www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at – 20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Background | E2 protein plays a central role in the viral life cycle as it regulates both transcription and replication of the viral genome. Can either activate or repress transcription. The E1-E2 protein complex binds to the origin of DNA replication activating replication of the BPV |
| References |
Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, Langel Ü, and Ustav M. 1999. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. 73: 4670-77. Kurg R, Tekkel H, Abroi A, and Ustav M. 2006. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. 80(22): 11218-25 |
| Limitations | This
product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
Figure 1. Western Blot testing of anti-BPV E2 monoclonal antibody (3F12). 2 ng of purified recombinant E2 protein was loaded.
A.
B.
Figure 2. Immunofluorescence testing of anti-BPV E2 monoclonal antibody (3F12). Assay was carried out with U2OS cells expressing BPV E2 protein. Antibody concentration of 1 µg/ml was used. Nuclei were stained by DAPI. Fluorescence was measured at (A) 460 nm and 488 nm wavelengths or (B) 488 nm only.