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Figure 1.Coomassie-stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike. 4-12% gradient gel is used for analysis. Lane 1.3 µg SARS-CoV-2 Trimeric Spike (-DTT). Lane 2. Protein marker (Smobio). Lane 3. 1.3 µg SARS-CoV-2 Trimeric Spike (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC for final product after 3 freeze-thaw cycles.
Catalogue #
P-309-100
Name
SARS-CoV-2 Trimeric Spike
Description
Protein contains amino acids 14-1211, plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain.
Uniprot ID
P0DTC2
Source
Human
MW
417.33 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Purification
Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
Purity
>95%
Concentration
1mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
QC
SDS-PAGE, analytical SEC
Shipping
Shipped on dry ice
Storage
Store at -70°C. Avoid multiple freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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