Search from website
Search from website
Figure 1. Simply Blue Safe stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike Omicron BA.5. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg SARS-CoV-2 Trimeric Spike Omicron BA.5 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Trimeric Spike Omicron BA.5 (+DTT)
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet RED96e analysis of SARS-CoV-2 Trimeric Spike Omicron protein binding to the ACE2 receptor.Catalogue #
P-382-100
Name
SARS-CoV-2 Trimeric Spike Omicron BA.5
Description
Protein contains amino acids 14-1211, T19I, L24S, del25/27, del69-70, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS).
MW
410.252 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Kd:
5.64E-12 (measured against ACE2 receptor)
Purification
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity
>80%
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
QC
Simply Blue Safe stained SDS-PAGE, analytical SEC, Octet binding to ACE2 receptor
Shipping
Shipped on dry ice
Storage
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
© 2023 Icosagen
