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Figure 1. Simply Blue stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike Indian B.1.617.1 . 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg SARS-CoV-2 Trimeric Spike Indian B.1.617.1 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Trimeric Spike Indian B.1.617.1 (+DTT).
Figure 2. HPLC analytical SEC for final product.
Figure 3. HPLC analytical SEC after 3 freeze-thaw cycles.
Figure 4. Octet Red96e analysis of SARS-CoV-2 Trimeric Spike Indian B.1.617.1 binding to human ACE2 receptor.
Catalogue #
P-352-100
Name
SARS-CoV-2 Trimeric Spike Indian B.1.617.1 (kappa)
Description
Protein contains amino acids 14-1211 and following mutations: G142D, E154K, L452R, E484Q, D614G, P681R. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS).
Uniprot ID
P0DTC2
MW
410.77 kDa
Host
CHO-based cell line (expressed by QMCF Technology)
Purification
Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
Purity
>95%
Concentration
1 mg/ml
Buffer
PBS pH 7.4
Endotoxine
NA
QC
SDS-PAGE, analytical SEC, Octet binding to ACE2 receptor
Shipping
Shipped on dry ice
Storage
Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.
This product is for research use only
TECHNICAL ASSISTANCE
Please refer any technical questions to
technical.support@icosagen.com
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